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Advancement of the Acetylene Inhibition Technique Using Time Series Analysis on Air-Dried Floodplain Soils to Quantify Denitrification Potential

机译:使用时间序列分析对风干泛洪叶土壤的乙炔抑制技术的推进,以量化脱氮潜力

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Denitrification in floodplain soils is one key process that determines the buffering capacity of riparian zones in terms of diffuse nitrate pollution. One widely used approach to measure the denitrification potential is the acetylene inhibition technique that requires fresh soil samples. We conducted experiments with air-dried soils using a time series analysis to determine the optimal rewetting period. Thus, air-dried soil samples from six different floodplain areas in Germany were rewetted for 1 to 13days to 100% water-filled pore space. We analyzed nitrogen accumulated as N 2 O in the top of anaerobic flasks with and without acetylene by gas chromatography after four hours of incubation. We observed an overall optimal rewetting of at least seven days for complete denitrification. We also saw the strong influence of pH and field capacity on the denitrification product ratio; in soils with pH 7, we hardly assumed complete denitrification, whereas the treatments with pH 7 achieved stable values after seven days of rewetting. This advanced method provides the opportunity to carry out campaigns with large soil sample sizes on the landscape scale, as samples can be stored dry until measurements are taken.
机译:洪泛区土壤中的反硝化是在弥漫硝酸盐污染方面确定河岸区的缓冲能力的一个关键过程。一种广泛使用的方法来测量脱硝电位是需要新鲜土壤样品的乙炔抑制技术。我们使用时间序列分析进行了空气干燥的空气干燥的实验,以确定最佳再润湿期。因此,德国六个不同洪泛区区域的风干土壤样品再次重新润湿1至13天至100%充满水的孔隙空间。在孵育4小时后,通过通过气相色谱分析在厌氧烧瓶顶部中累积为N 2 O的N 2 O积聚的氮气。我们观察到完全最佳再润湿至少七天以进行完全反硝化。我们还看到了pH和现场容量对脱硝产品比的强烈影响;在pH <7的土壤中,我们几乎没有假设完全的反硝化,而用pH> 7的治疗在重新灌注七天后达到稳定的值。这种先进的方法提供了在景观量表上进行大型土样样本尺寸的运动,因为样品可以储存干直至测量。

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