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Development and optimization of an immunoassay for the detection of Hg(II) in lake water

机译：湖水中HG（II）检测的免疫测定的开发和优化

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In this paper, an indirect competitive enzyme‐linked immunosorbent assay (IC‐ELISA) has been developed and optimized to detect Hg(II) in tap water and lake water based on a monoclonal antibody (mAb‐A24). Some stabilizing additives (Gelatin, bovine serum albumin [BSA], polyvinyl alcohol [PVA], and polyvinyl pyrrolidone [PVP]) and surfactant (Tween‐20) have been investigated thoroughly in the optimization process. Under the optimal condition, the 50% half maximal inhibitory concentration (IC 50 ) and limit of detection (LOD) were 1.68 and 0.079?ng/ml, respectively. These anti‐Hg mAbs also have some affinity with methyl mercury (CH 3 Hg) and with no cross‐reactivity with other thirteen metal ions. The developed method has shown satisfactory recovery of Hg(II), ranged between 91% and 116%, from tap water and lake water. Therefore, this immunoassay can be used to detect trace Hg(II) in environment water.
机译：本文已经开发并优化了间接竞争性酶联免疫吸附测定（IC-ELISA）以基于单克隆抗体（MAB-A24）检测自来水和湖水中的Hg（II）。已经在优化过程中彻底研究了一些稳定的添加剂（明胶，牛血清白蛋白[BSA，聚乙烯醇[PVA]，聚乙烯醇[PVA]和聚乙烯吡咯烷酮[PVP]）和表面活性剂（TWEEN-20）。在最佳条件下，50％半的最大抑制浓度（IC 50）和检测极限分别为1.68和0.079≤ng/ ml。这些抗HG mAb也具有与甲基汞（CH 3 HG）的亲和力，并且与其他十三金属离子没有交叉反应性。开发方法显示出令人满意的Hg（II）恢复，范围为91％至116％，来自自来水和湖水。因此，该免疫测定可用于检测环境水中的痕量HG（II）。

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