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首页> 外文期刊>Frontiers in Microbiology >Production of Bacterial Cellulose by Gluconacetobacter hansenii Using Corn Steep Liquor As Nutrient Sources
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Production of Bacterial Cellulose by Gluconacetobacter hansenii Using Corn Steep Liquor As Nutrient Sources

机译：通过<斜视>葡聚糖杆菌的细菌纤维素的生产使用玉米陡液作为营养来源

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Cellulose is mainly produced by plants, although many bacteria, especially those belonging to the genus Gluconacetobacter , produce a very peculiar form of cellulose with mechanical and structural properties that can be exploited in numerous applications. However, the production cost of bacterial cellulose (BC) is very high to the use of expensive culture media, poor yields, downstream processing, and operating costs. Thus, the purpose of this work was to evaluate the use of industrial residues as nutrients for the production of BC by Gluconacetobacter hansenii UCP1619. BC pellicles were synthesized using the Hestrin–Schramm (HS) medium and alternative media formulated with different carbon (sugarcane molasses and acetylated glucose) and nitrogen sources [yeast extract, peptone, and corn steep liquor (CSL)]. A jeans laundry was also tested. None of the tested sources (beside CSL) worked as carbon and nutrient substitute. The alternative medium formulated with 1.5% glucose and 2.5% CSL led to the highest yield in terms of dry and hydrated mass. The BC mass produced in the alternative culture medium corresponded to 73% of that achieved with the HS culture medium. The BC pellicles demonstrated a high concentration of microfibrils and nanofibrils forming a homogenous, compact, and three-dimensional structure. The biopolymer produced in the alternative medium had greater thermal stability, as degradation began at 240°C, while degradation of the biopolymer produced in the HS medium began at 195°C. Both biopolymers exhibited high crystallinity. The mechanical tensile test revealed the maximum breaking strength and the elongation of the break of hydrated and dry pellicles. The dry BC film supported up to 48 MPa of the breaking strength and exhibited greater than 96.98% stiffness in comparison with the hydrated film. The dry film supported up to 48 MPa of the breaking strength and exhibited greater than 96.98% stiffness in comparison with the hydrated film. The values obtained for the Young’s modulus in the mechanical tests in the hydrated samples indicated low values for the variable rigidity. The presence of water in the interior and between the nanofibers of the hydrated BC only favored the results for the elasticity, which was 56.37% higher when compared to the dry biomaterial.
机译：纤维素主要由植物产生，尽管许多细菌，尤其是属于葡糖杆菌属的细菌，产生具有非常奇特形式的纤维素，其具有可以在许多应用中被利用的机械和结构性。然而，细菌纤维素（BC）的生产成本对使用昂贵的培养基，较差的产量，下游加工和运营成本非常高。因此，这项工作的目的是评估工业残留物的使用作为葡聚糖杆菌UCP1619生产BC的营养素。使用用不同碳（甘蔗糖蜜和乙酰化葡萄糖）和氮源[酵母提取物，蛋白胨和玉米陡液（CSL）配制的Hestrin-Schramm（HS）培养基和替代介质合成BC颗粒。牛仔裤洗衣店也得到了测试。没有测试的来源（CSL旁边）作为碳和营养替代品。配方用1.5％葡萄糖和2.5％CSL配制的替代介质导致干燥和水合质量方面的最高收益率。在替代培养基中产生的BC质量与HS培养基达到的73％相当于73％。 BC颗粒显示出高浓度的微纤维和纳米纤维，形成均匀，紧凑，三维结构。在替代介质中产生的生物聚合物具有更大的热稳定性，因为降解在240℃下开始，而HS培养基中产生的生物聚合物的降解开始于195℃。两种生物聚合物表现出高结晶度。机械拉伸试验揭示了水合和干囊突破的最大断裂强度和伸长率。与水合膜相比，干BC膜负载高达48MPa的断裂强度，并且与水合膜相比，刚度大于96.98％。与水合膜相比，干膜支撑着高达48MPa的断裂强度，并且与水合膜相比表现出大于96.98％的刚度。在水合样品中的机械测试中为杨氏模量获得的值表示可变刚度的低值。水合BC的内部和纳米纤维之间的水的存在仅利于弹性的结果，与干生物材料相比，该弹性较高56.37％。

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