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首页> 外文期刊>Frontiers in Neuroanatomy >Monosynaptic Input Mapping of Diencephalic Projections to the Cerebrospinal Fluid-Contacting Nucleus in the Rat
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Monosynaptic Input Mapping of Diencephalic Projections to the Cerebrospinal Fluid-Contacting Nucleus in the Rat

机译：Diencephalic突起对大鼠脑脊液接触细胞核的单突触性输入映射

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Objective : To investigate the projections the cerebrospinal fluid-contacting (CSF-contacting) nucleus receives from the diencephalon and to speculate on the functional significance of these connections. Methods : The retrograde tracer cholera toxin B subunit (CB) was injected into the CSF-contacting nucleus in SD rats according to the experimental formula of the stereotaxic coordinates. Animals were perfused 7–10 days after the injection, and the diencephalon was sliced at 40 μm with a freezing microtome. CB-immunofluorescence was performed on all diencephalic sections. The features of CB-positive neuron distribution in the diencephalon were observed with a fluorescence microscope. Results : The retrograde labeled CB-positive neurons were found in the epithalamus, subthalamus, and hypothalamus. Three functional diencephalic areas including 43 sub-regions revealed projections to the CSF-contacting nucleus. The CB-positive neurons were distributed in different density ranges: sparse, moderate, and dense. Conclusion : Based on the connectivity patterns of the CSF-contacting nucleus that receives anatomical inputs from the diencephalon, we preliminarily assume that the CSF-contacting nucleus participates in homeostasis regulation, visceral activity, stress, emotion, pain and addiction, and sleeping and arousal. The present study firstly illustrates the broad projections of the CSF-contacting nucleus from the diencephalon, which implies the complicated functions of the nucleus especially for the unique roles of coordination in neural and body fluids regulations.
机译：目的：探讨脑脊液接触（CSF接触）核从DiETyphalon接受并推测这些连接的功能意义。方法：根据立体坐标的实验式在SD大鼠中将逆行示踪剂霍乱毒素B子单元（CB）注入CSF接触核中。将动物灌注在注射后7-10天，并且Diencephalon以40μm切成40μm，冷冻切片体。在所有Diencephalic部分进行CB-免疫荧光。用荧光显微镜观察到Diencephalon中CB阳性神经元分布的特征。结果：逆行标记的CB阳性神经元在上皮阳性，地区和下丘脑中发现。包括43个子区域的三个功能性Diencephalic区域揭示了CSF接触核的突起。 CB阳性神经元以不同的密度范围分布：稀疏，中等和密集。结论：基于接受Diencephalon的解剖学输入的CSF接触核的连通性模式，我们初步假设CSF接触核参与稳态调节，内脏活动，压力，情感，疼痛和成瘾，以及睡眠和唤醒。本研究首先说明了CSF接触核来自DiETyphalon的宽突起，这意味着核的复杂功能，特别是对于在神经和体液法规中的协调的独特作用。

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