首页> 外文期刊>Frontiers in Physiology >Growth and Development of Helicoverpa armigera (Lepidoptera: Noctuidae) Larvae Infected by Heliothis virescens ascovirus 3i (HvAV-3i)
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Growth and Development of Helicoverpa armigera (Lepidoptera: Noctuidae) Larvae Infected by Heliothis virescens ascovirus 3i (HvAV-3i)

机译:<斜视> Helicoverpa Armigera (鳞翅目)(鳞翅目)幼虫(Heliothis Virescens Ascovirus 3i(HVAV-3I)感染

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Although the cotton bollworm Helicoverpa armigera has traditionally been controlled by application of chemical pesticides, chemical control selects for resistance, pollutes the environment, and endangers human health. New methods for controlling H. armigera are therefore needed. Heliothis virescens ascovirus 3i (HvAV-3i) is a recently identified virus of the lepidopteran larvae. We tested the effects of HvAV-3i on H. armigera larvae following oral ingestion of HvAV-3i-containing hemolymph (about 1.0 × 10 ~(10) virus genome copies per larvae) and following injection of HvAV-3i-containing hemolymph by insertion of a needle. Following oral ingestion, first-instar to fifth-instar larvae grew and developed normally. Following needle injection, in contrast, the corrected mortality of third and fourth instars was 88.9 ± 2.1 and 93.7 ± 3.4%, respectively. Food intake was significantly lower for larvae injected with virus-containing hemolymph than with virus-free hemolymph. Larvae injected with virus-containing hemolymph had extended survival times and could not complete the pre-pupal stage. These results indicate that inoculation of HvAV-3i via needle injection, but not via oral ingestion, significantly reduced the growth and development of H. armigera larvae.
机译:虽然棉铃虫Helicoverpa Armigera传统上是通过应用化学农药的应用,但化学对照选择抗性,污染环境,危及人类健康。因此需要用于控制H. armigera的新方法。 Heliothis virescens ascovirus 3i(hvav-3i)是鳞翅目幼虫最近鉴定的病毒。在口服摄入HVAV-3i血淋巴(约1.0×10〜(10)病毒基因组拷贝每只幼虫时,并通过插入注射含HVAV-3I含有HVAV-3I的血淋巴,对HVAV-3I对H. Armigera幼虫的影响针头。口腔摄入后,第一龄至第五套幼虫正常发展和发展。在针刺后,相比之下,第三和第四龄的校正死亡率分别为88.9±2.1和93.7±3.4%。对于含有病毒血淋巴的幼虫而不是无病毒血淋巴,食物摄入量显着降低。幼虫注射含有病毒的血淋巴的存活时间延长,无法完成蛹前阶段。这些结果表明,通过针注射接种HVAV-3i,但不通过口服摄入,显着降低了H.Armigera幼虫的生长和发育。

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