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Data of in vitro synthesized dsRNAs on growth and development of Helicoverpa armigera

机译：体外的数据：Italic> in合成的Dsrnas对 helicoverpa armigera

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The data presented in this article is related to the research article “RNAi of selected candidate genes interrupts growth and development of Helicoverpa armigera ” (Chikate et al., 2016) . RNA interference (RNAi) is emerging as a potent insect pest control strategy over current methods and their resistance by pest. In this study we tested 15 different in vitro synthesized dsRNAs for gene silencing in Helicoverpa armigera . These dsRNAs were specific against H. armigera enzymes/proteins such as proteases like trypsins ( HaTry2 , 3, 4 and 6 ), chymotrypsin ( HaChy4 ) and cysteine proteases such as cathepsin ( HaCATHL ); glutathione S-transferases ( HaGST1a, 6 and 8 ); esterases ( HaAce4, HaJHE ); catalase ( HaCAT ); super-oxide-dismutase ( HaCu/ZnSOD ); fatty acid binding protein ( HaFabp ) and chitin deacetylase ( HaCda5b ). These dsRNAs were fed to second instar larvae at an optimized dose (60 μg/day) for 3 days separately. Effects of dsRNA feeding were observed in terms of larval mass gain, percentage mortality and phenotypic abnormalities in later developmental stages of H. armigera . These findings might provide potential new candidates for designing sequence-specific dsRNA as pesticide in crop protection.
机译：本文提出的数据与研究制品“选定的候选基因RNAi中断Helicoverpa Armigera的生长和发展”（Chikate等，2016）。 RNA干扰（RNAi）作为电流方法的有效昆虫防治策略以及害虫的抵抗力。在这项研究中，我们在Helicoverpa Armigera中测试了15种不同的体外合成的DSRNA用于基因沉默。这些DSRNA对H. armigera酶/蛋白质如胰蛋白酶（Hacry2,3,4和6），胰凝乳素（Hachy4）和半胱氨酸蛋白酶（如组织蛋白酶（Hacathl）等蛋白酶）特异性。谷胱甘肽S-转移酶（Hagst1a，6和8）;酯酶（HAACE4，HAJHE）;过氧化氢酶（HACAT）;超氧化物 - 歧化酶（HACU / ZnSOD）;脂肪酸结合蛋白（HAFABP）和甲壳素脱乙酰酶（HACDA5B）。将这些DSRNA分别以优化剂量（60μg/天）送入第二龄幼虫3天。在幼虫质量增益，H. Armigera后期发育阶段的幼虫大众化，百分比死亡率和表型异常方面观察到DSRNA饲养的影响。这些发现可能为在作物保护中设计序列特异性DSRNA的潜在新候选者。

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