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首页> 外文期刊>Frontiers in Plant Science >Morphological Structure and Transcriptome Comparison of the Cytoplasmic Male Sterility Line in Brassica napus (SaNa-1A) Derived from Somatic Hybridization and Its Maintainer Line SaNa-1B
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Morphological Structure and Transcriptome Comparison of the Cytoplasmic Male Sterility Line in Brassica napus (SaNa-1A) Derived from Somatic Hybridization and Its Maintainer Line SaNa-1B

机译：细胞质雄性不育线在体细胞杂交及其维护系SANA-1B中的细胞质雄性不育线中的形态学结构和转录组细胞质雄性不育系比较

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SaNa-1A is a novel cytoplasmic male sterility (CMS) line in Brassica napus derived from progenies of somatic hybrids between B. napus and Sinapis alba , and SaNa-1B is the corresponding maintainer line. In this study, phenotypic differences of floral organs between CMS and the maintainer lines were observed. By microscope observation in different anther developmental stages of two lines, we found the anther development in SaNa-1A was abnormal since the tetrad stage, and microspore development was ceased during the uninucleate stage. Transcriptomic sequencing for floral buds of sterile and fertile plants were conducted to elucidate gene expression and regulation caused by the alien chromosome and cytoplasm from S. alba . Clean tags obtained were assembled into 195,568 unigenes, and 7811 unigenes distributed in the metabolic and protein synthesis pathways were identified with significant expression differences between two libraries. We also observed that genes participating in carbon metabolism, tricarboxylic acid cycle, oxidative phosphorylation, oxidation–reduction system, pentatricopeptide repeat, and anther development were downregulated in the sterile line. Some of them are candidates for researches on the sterility mechanism in the CMS material, fertility restoration, and improvement of economic traits in the maintainer line. Further research on the tags with expressional specificity in the fertile line would be helpful to explore desirable agronomic traits from wild species of rapeseed.
机译：SANA-1A是一种新型细胞质雄性不育（CMS）在Brassica Napus中衍生自B. Napus和Sinapis Alba之间的体细胞杂种的后代，而SANA-1B是相应的维护系列。在该研究中，观察到CMS与维护系之间的花粉的表型差异。通过显微镜观察在不同的制剂发育阶段两条线，我们发现SANA-1A的花药发育异常，因为四阶段，并且在无核阶段停止了微孔发育。进行了对无菌和肥沃植物的花芽的转录组测序，以阐明由S. Alba的外星染色体和细胞质引起的基因表达和调节。将获得的清洁标签组装成195,568个unigenes，并在代谢和蛋白质合成途径中分布的7811个未在两个文库之间的表达差异中鉴定出来。我们还观察到参与碳代谢，三羧酸循环，氧化磷酸化，氧化还原系统，五氢肽重复和花药的基因在无菌线中下调。其中一些是关于CMS材料，生育恢复和维护系中经济特征的不耐药机制的研究的候选者。进一步研究了肥沃的植物中表达特异性的标签将有助于探讨来自油菜野生种类的理想农艺性状。

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