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首页> 外文期刊>Functional Foods In Health And Disease >Preliminary study on the In-Vitro Susceptibility of Mycobacterium tuberculosis Isolates to Virgin Coconut Oil
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Preliminary study on the In-Vitro Susceptibility of Mycobacterium tuberculosis Isolates to Virgin Coconut Oil

机译：结核分枝杆菌分离株与尾椰子油的体外易感性初步研究

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Background: Tuberculosis remains a major public health threat. Studies have shown that medium chain fatty acids (MCFA), such as, those found in Virgin Coconut Oil (VCO), possess activity against a wide range of microorganisms including?Mycobacterium tuberculosis. The goal of the study was to determine the in-vitro susceptibility of?M. tuberculosis (TB) isolates to two commercial brands of VCOs each containing different amounts of lauric acid and produced?by two different extraction processes. Two brands of VCO were tested for their anti-TB actions compared to pure lauric acid and a negative control. Two replicate runs were done. VCO samples were inoculated with 0.1 mL of the H37RV strain of M. tuberculosis and incubated at 370C with shaking for 5 days. After incubation, the mixtures were diluted 10-fold up to 10-4?concentration to obtain countable colonies using Middlebrook 7H10 agar plates and Lowenstein-Jensen (L-J) slants. Colony growths of M. tuberculosis were examined in each dilution every week for three to eight weeks.
机译：背景：结核病仍然是一个主要的公共卫生威胁。研究表明，中链脂肪酸（MCFA），例如在维尔京椰子油（VCO）中发现的那些，具有针对各种微生物的活性，包括结核分枝杆菌。该研究的目标是确定ΔM的体外易感性。结核病（TB）分离到两种商业品牌的VCO，每个商业品牌含有不同量的月桂酸并产生α并产生两种不同的提取过程。与纯的月桂酸和阴性对照相比，测试了两个品牌的VCO品牌的抗TB动作。完成了两个复制运行。将VCO样品接种含量为0.1mL结核的H37RV菌株，并在370℃下振荡孵育5天。孵育后，将混合物稀释10倍至10-4倍浓度以获得使用中间琼脂平板和Lowenstein-jensen（L-J）倾斜来获得可计数的菌落。每周在每周稀释时检查菌落的殖民地生长3至8周。

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