...
  • 主题
高级搜索  >
历史搜索 清空历史
首页> 外文期刊>Frontiers in Bioengineering and Biotechnology >Short Hairpin RNAs for Strand-Specific Small Interfering RNA Production
【24h】

Short Hairpin RNAs for Strand-Specific Small Interfering RNA Production

机译：用于股线特异性小干扰RNA生产的短发夹RNA

获取原文
           
页面导航
  • 摘要
  • 著录项
  • 相似文献
  • 相关主题

摘要

RNA interference (RNAi) is an effective mechanism for inhibiting gene expression at the post-transcriptional level. Expression of a messenger RNA (mRNA) can be inhibited by a ~22-nucleotide (nt) small interfering (si)RNA with the corresponding reverse complementary sequence. Typically, a duplex of siRNA, composed of the desired siRNA and a passenger strand, is processed from a short hairpin RNA (shRNA) precursor by Dicer. Subsequently, one strand of the siRNA duplex is associated with Argonaute (Ago) protein for RNAi. Although RNAi is widely used, the off-target effect induced by the passenger strand remains a potential problem. Here, based on current understanding of endogenous precursor microRNA (pre-miRNA) hairpins, called Ago-shRNA and m7G-capped pre-miRNA, we discuss the principles of shRNA designs that produce a single siRNA from one strand of the hairpin.
机译：RNA干扰（RNAi）是抑制在转录后水平的基因表达的有效机制。通过相应的反向互补序列的〜22核苷酸（NT）小干扰（Si）RNA可以抑制信使RNA（mRNA）的表达。通常，由所需siRNA和乘客链组成的siRNA的双链体由加入剂从短发夹RNA（ShRNA）前体处理。随后，SiRNA双链链的一条链与RNAi的Argonaute（前）蛋白质有关。虽然RNAi被广泛使用，但乘客链引起的偏离目标效果仍然是潜在的问题。这里，基于目前对内源性前体MicroRNA（前miRNA）发夹的最新理解，调用ay-shRNA和M7G-lecapped预先生，我们讨论了从发夹的一股产生单个siRNA的shRNA设计原理。

著录项

相似文献

  • 外文文献
  • 中文文献
  • 专利
获取原文

客服邮箱：kefu@zhangqiaokeyan.com

京公网安备：11010802029741号 ICP备案号：京ICP备15016152号-6 六维联合信息科技 (北京) 有限公司©版权所有

  • 客服微信

  • 服务号