...
  • 主题
高级搜索  >
历史搜索 清空历史
首页> 外文期刊>Frontiers in Veterinary Science >Potential Role of SLC5A8 Expression in the Etiology of Subacute Ruminal Acidosis
【24h】

Potential Role of SLC5A8 Expression in the Etiology of Subacute Ruminal Acidosis

机译：SLC5A8表达在亚急性瘤胃中毒病因中的潜在作用

获取原文
           
页面导航
  • 摘要
  • 著录项
  • 相似文献
  • 相关主题

摘要

Rumen fluid of cows with subacute ruminal acidosis (SARA) have high concentrations of short chain fatty acids (SCFA). However, the mechanism of SCFA accumulation is unknown. The solute-linked carrier 5a8 (SLC5A8) plays a key role in the transportation and absorption of SCFA in intestinal epithelium. The objective of the current study was to investigate (1) SLC5A8 gene and protein expression in various parts of the bovine gastrointestinal tract, (2) the effect of SCFA on SLC5A8 expression in rumen epithelial cells, and (3) SLC5A8 gene and protein expression in SARA and healthy cows. A total of 10 dairy cows, five in each group, SARA cows and control cows were 84 ± 26 days in milk and in their 2-4 parity. Three of adult cows and three calves (one-day-old, female, 45-50 kg, healthy, fasting) were chosen to collect total ten sections of digestive tract, from rumen to rectum, then bovine ruminal epithelial cells were isolated from three calves. Gene and protein expression of SLC5A8 was detected in all tested regions of gastrointestinal tract in calves and adult cows by western blot and quantitative real-time PCR, and were both highest in the rumen. Gene and protein expression of SLC5A8 was more than 50% lower in the rumen epithelium of SARA vs. Control cows and was partly restored after therapy of SARA cows. Compared with SCFA concentrations typical for control cows (60 mM acetate, 30 mM propionate and 20 mM butyrate), gene and protein expression of SLC5A8 in rumen epithelium was lower at elevated SCFA concentrations typical for SARA cows (90 mM acetate, 40 mM propionate and 30 mM butyrate); specifically for elevated concentrations of propionate or butyrate. In contrast, elevated concentrations of acetate increased gene and protein expression of SLC5A8 in rumen epithelium. In conclusion, the elevated concentrations of propionate and butyrate inhibit ruminal absorption of SCFA via downregulation of SLC5A8 in SARA cows, the expression of SLC5A8 plays important role in the etiology of SARA.
机译：具有亚急性瘤胃酸中毒（SARA）的奶牛的瘤胃液具有高浓度的短链脂肪酸（SCFA）。然而，SCFA积累的机制是未知的。溶质连接的载体5A8（SLC5A8）在肠上皮中的运输和吸收中起着关键作用。目前研究的目的是研究（1）SLC5A8基因和蛋白质表达在牛胃肠道的各个部分中，（2）SCFA对瘤胃上皮细胞中SLC5A8表达的影响，（3）SLC5A8基因和蛋白质表达在萨拉和健康的奶牛。共有10个乳制品奶牛，每组五个，牛奶和控制奶牛在牛奶中的84±26天，在2-4个平价。选择三个成人奶牛和三个小牛（一天，女性，45-50公斤，健康，禁食），从瘤胃到直肠来收集总消化道的十个部分，然后从三个中分离牛瘤胃上皮细胞小牛。通过蛋白质印迹和定量实时PCR在牛犊和成人奶牛的所有测试区域中检测到SLC5A8的基因和蛋白表达，并在瘤胃中均有最高。 SARA对照牛的瘤胃上皮细胞上皮的基因和蛋白质表达在瘤胃上皮瘤中较低，并且在萨拉奶牛治疗后部分恢复。与对照奶牛的典型SCFA浓度相比（乙酸盐60mm，丙酸盐30mm丁酸丁酸丁酸丁酸丁酸丁酸丁酸丁酸丁酸丁酸盐，SLC5A8的基因和蛋白质表达在SARA牛的典型SCFA浓度下较低（乙酸乙酯，40mM丙酸盐40mM和40mM 30毫米丁酸盐）;具体用于升高浓度的丙酸盐或丁酸盐。相反，胃肠上皮中SLC5a8的醋酸盐增加的升高浓度增加，含量高。总之，通过在Sara牛中的SLC5a8的下调升高的丙酸盐和丁酸酯抑制SCFA的瘤胃吸收，SLC5A8的表达在SARA的病因中起着重要作用。

著录项

相似文献

  • 外文文献
  • 中文文献
  • 专利
获取原文

客服邮箱：kefu@zhangqiaokeyan.com

京公网安备：11010802029741号 ICP备案号：京ICP备15016152号-6 六维联合信息科技 (北京) 有限公司©版权所有

  • 客服微信

  • 服务号