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首页> 外文期刊>Medical principles and practice: international journal of the Kuwait University, Health Science Centre >Phenotypic and Genotypic Characterization of ESBL-, AmpC-, and Carbapenemase-Producing Klebsiella pneumoniae and Escherichia coli Isolates
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Phenotypic and Genotypic Characterization of ESBL-, AmpC-, and Carbapenemase-Producing Klebsiella pneumoniae and Escherichia coli Isolates

机译：Esbl-，Ampc-和碳结构酶的表型和基因型表征产生的肺炎氏蛋白酶菌和大肠杆菌分离物

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Objectives: Drug resistance among gram-negative bacteria is a worldwide challenge. Due to the importance of drug-resistant Klebsiella pneumoniae and Escherichia coli strains in hospital-acquired infections, we aimed to determine the phenotypic and genotypic characteristics of ESBL-, AmpC-, and carbapenemase-producing isolates obtained from hospitalized patients in Tehran and Ilam (Iran). Materials and Methods: In total, 90 K. pneumoniae isolates and 65 E. coli isolates were collected from various infections. Phenotypic identification of bacterial isolates was performed using standard methods. Phenotypic screening of ESBL, AmpC, and carbapenemase enzymes was carried out. Detection of ESBL, AmpC, and carbapenemase genes was also performed by the PCR method. Results: Phenotypic detection tests showed that 36 (40%) K. pneumoniae and 23 (35.4%) E. coli isolates were ESBL producers. Moreover, 18 (20%) and 6 (9.2%) K. pneumoniae and E. coli isolates were AmpC producers, respectively. Modified Hodge test results indicated that 39 (43.3%) K. pneumoniae and 18 (27.7%) E. coli isolates produced carbapenemase. Molecular tests showed that 40% of K. pneumoniae and 36.9% of E. coli isolates were ESBL positive. AmpC was detected in 24.4 and 13.8% of K. pneumoniae and E. coli isolates. Carbapenemase was detected in 34 (37.8%) K. pneumoniae and 13 (20%) E. coli isolates. Conclusion: In this study, 3 K. pneumoniae isolates simultaneously carried ESBL, AmpC, and carbapenemase genes. Up-to-date strategies such as combination therapy or utilization of new antimicrobial agents might help to combat such drug-resistant organisms.
机译：目的：革兰氏阴性细菌之间的耐药性是全球挑战。由于耐药性Klebsiella肺炎和大肠杆菌菌株在医院获得的感染中的重要性，我们旨在确定从德黑兰和伊兰州住院患者获得的ESBL-，AMPC和Carbapenemase的分离株的表型和基因型特征（伊朗）。材料和方法：总共90 k。肺炎肺炎分离物和65大肠杆菌分离株从各种感染中收集。使用标准方法进行细菌分离株的表型鉴定。进行ESBL，AMPC和碳比酶的表型筛选。通过PCR方法还进行ESBL，AMPC和碳碱酶基因的检测。结果：表型检测试验表明，36（40％）K.Pneumoniae和23（35.4％）大肠杆菌分离物是ESBL生产商。此外，18（20％）和6（9.2％）K.Pneumoniae和大肠杆菌分离株分别为AMPC生产商。改进的Hodge测试结果表明，39（43.3％）K.Pneumoniae和18（27.7％）大肠杆菌分离株产生的碳结构酶。分子试验表明，40％的K.Pneumoniae和36.9％的大肠杆菌分离物的ESBL阳性。在24.4和13.8％的K.Pneumoniae和大肠杆菌分离株中检测到AMPC。在34（37.8％）K.Pneumoniae和13（20％）大肠杆菌分离物中检测到碳丙糖酶。结论：在本研究中，3K.肺炎群分离物同时携带ESBL，AMPC和碳碱酶基因。新抗菌剂的联合治疗或利用等最新策略可能有助于打击这种抗药性生物。

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