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Modulation of Pasteurella multocida capsular polysaccharide during growth under ironrestricted conditions and in vivo

机译：在铁人营造条件下和体内生长过程中Pasteurella multocada多糖的调节

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Summary: Addition of the iron chelators 2,2-dipyridyl, deferoxamine mesylate or apotransferrin to culture media affected the composition and the morphology of Pasteurella multocida cells. Cells grown under iron-restricted conditions expressed iron-regulated proteins and, in addition, iron deprivation markedly reduced the amount of capsular material covering the cells of P. multocida. The addition of iron neutralized the effect of these chelators on capsule production. Cells of P. multocida grown under iron-restricted conditions were more labelled by gold particles coated with polymyxin which is known to interact with the lipid A-core region of lipopolysaccharides, and showed increased affinity for porcine respiratory tract mucus than cells grown under iron-sufficient conditions. Bacterial cells grown in vivo in peritoneal chambers in rats were also only covered by a thin layer (15-20 nm) of capsular material. Although the capsule is believed to be an important virulence factor, our data indicate that under iron-restricted conditions, such as those encountered in vivo, P. multocida may not be heavily encapsulated.
机译：发明内容：加入铁螯合剂2,2-双吡啶基，Deiferoxamine甲磺酸酯或培养培养基的疏水素，影响了巴斯氏菌多型细胞的组成和形态。在铁限制条件下生长的细胞表达了铁调节蛋白，并且另外，铁剥夺显着降低了覆盖P.多偶的细胞的囊性材料的量。添加铁中和这些螯合剂对胶囊生产的影响。在铁限制条件下涂覆在铁限制条件下的多型细胞被涂覆的金属蛋白的金颗粒标记，已知与脂多糖的脂质A芯区域相互作用，并且表明对猪呼吸道粘液的亲和力增加于铁下的细胞 - 充分条件。大鼠腹膜室中生长的细菌细胞也仅被薄层（15-20nm）的荚膜材料覆盖。虽然胶囊被认为是一个重要的毒力因素，但我们的数据表明，在铁限制条件下，例如在体内遇到的那些，P. Multocida可能不会受到大量封装。

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