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首页> 外文期刊>Microbiology >Isolation and characterization of a hydrogen peroxide resistant mutant of Bacillus subtilis
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Isolation and characterization of a hydrogen peroxide resistant mutant of Bacillus subtilis

机译：枯草芽孢杆菌过氧化氢耐氧化官突变体的分离与表征

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Summary: A mutant of Bacillus subtilis has been isolated by continuous selection in increasing concentrations of H2O2. It grew with a doubling time of 85 min in minimal medium containing 150 mM H2O2, whereas the wild-type parent lysed in 100 mM H2O2. The mutant was also more resistant to organic peroxides than the wild-type. Further resistance to H2O2 could not be induced by pretreatment with low concentrations of the oxidant. The mutant synthesized a number of proteins at a much higher rate than the wild-type, including constitutive synthesis of all of the proteins which were induced by H2O2 in the wild-type. Four of these proteins were sequenced; three were identified as catalase and two subunits of alkyl hydroperoxide reductase. Two proteins whose synthesis was repressed in the mutant were sequenced, and one was identified as flagellin. The mutant grew as non-flagellated, partially septate, filaments of cells, and fragments of flagella were seen in the surrounding medium.
机译：发明内容：通过连续选择在增加H 2 O 2的浓度下进行芽孢杆菌突变体。它在含有150mM H 2 O 2的最小培养基中增长了85分钟的倍增时间，而野生型母体在100mM H 2 O 2中裂解。突变体也比野生型更抗有机过氧化物。通过用低浓度的氧化剂预处理不能诱导对H 2 O 2的进一步抗性。该突变体以比野生型更高的速率合成了多种蛋白质，包括所有蛋白质的组成性合成，其在野生型中由H 2 O 2诱导。这些蛋白质中的四种测序;将三种被鉴定为过氧化氢酶和烷基氢化氧化氧化物还原酶的两个亚基。测序在突变体中抑制其合成的两种蛋白质，并将一种被鉴定为鞭毛蛋白。突变体在未破坏，部分乙酸细胞，细胞的细胞，在周围培养基中看到鞭毛的片段。

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