首页> 外国专利> CHARACTERIZATION OF ITURIN HIGH-YIELDING UV MUTANT BACILLUS SUBTILIS AND THE IMPROVEMENT OF ITURIN PRODUCING BACILLUS SUBTILIS BY SITE-DIRECTED MUTAGENESIS

CHARACTERIZATION OF ITURIN HIGH-YIELDING UV MUTANT BACILLUS SUBTILIS AND THE IMPROVEMENT OF ITURIN PRODUCING BACILLUS SUBTILIS BY SITE-DIRECTED MUTAGENESIS

机译:定向定向诱变对ITURIN高产紫外突变芽孢杆菌球菌的鉴定及产生ITURIN的芽孢杆菌球菌的改良

摘要

The present invention relates to characteristics of high iturin-yielding UV mutant Bacillus subtilis, and a method for improving an inturin producing strain by site-directed mutagenesis. Specifically, as a result of primary and secondary UV treatments on a wild Bacillus subtilis strain, iturin yield is increased, and a part of mutagenesis and characteristics thereof are confirmed by the base sequence analysis of a secondarily UV-treated strain, so the strain having increased iturin yield by using a mutant-derived iturin biosynthesis gene is improved and can be used as a biological controlling agent, and the gene is used to transform a strain having different biological control activity, thereby being used in development of a novel strain.
机译:本发明涉及高产量尿素素的UV突变型枯草芽孢杆菌的特性,以及通过定点诱变来提高产产杜林素的菌株的方法。具体而言,通过对野生枯草芽孢杆菌菌株进行初次和二次UV处理,结果增加了iturin的产量,并通过对二次UV处理的菌株的碱基序列分析证实了部分诱变及其特性,因此该菌株具有通过使用衍生自突变体的叶绿素生物合成基因,提高了叶绿素产量的提高,并且可以用作生物防治剂,并且该基因用于转化具有不同生物防治活性的菌株,从而用于开发新菌株。

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