• 主题
高级搜索  >
历史搜索 清空历史
首页> 外文期刊>Molecular cytogenetics >The karyotype of Aegilops geniculata and its use to identify both addition and substitution lines of wheat
【24h】

The karyotype of Aegilops geniculata and its use to identify both addition and substitution lines of wheat

机译：Aegilops Geniculata的核型及其用于鉴定小麦的加法和替代线

获取原文
           
页面导航
  • 摘要
  • 著录项
  • 相似文献
  • 相关主题

摘要

BackgroundAegilops geniculata Roth (syn. Ae. ovata L, 2n?=?4x?=?28, genome formula UgMg) represents a source of potentially useful genetic variation of relevance to bread wheat improvement. The species is thought to represent a natural allotetraploid between the diploid Ae. umbellulata Zhuk. (U genome carrier) and Ae. comosa Sibth. et Sm. (M genome carrier) [1]. A number of genes conferring resistance to various diseases have been transferred from this species into bread wheat, notably Yr40 (resistance against stripe rust), Lr57 (leaf rust) [2], Sr53 (stem rust) [3] and Pm29 (powdery mildew) [4, 5]. Some accessions of Ae. geniculata have displayed high levels of water use efficiency [6], and the species overall exhibit a higher tolerance to moisture stress than any of the related species Ae. markgrafii (Greuter) K. Hammer, Ae. longissima Schweinf. & Muschl., Ae. searsii Feldman et Kislev ex K. Hammer or Ae. speltoides Tausch [7]. It also carries alleles at the genes encoding endosperm proteins which have been predicted to improve the end-use quality of bread wheat [8, 9].Genomic in situ hybridization (GISH) has been a very successful technique for discriminating between the chromosomes belonging to the various genomes represented in Triticeae species, while fluorescence in situ hybridization (FISH) tends to be used for identifying individual chromosomes. A FISH-based karyotype of Ae. geniculata has been established, employing the probe combination pSc119.2, Afa family repeats, pAs1 and pTa71 [10,11,12]. However, some segments of the Ae. geniculata genome lack any probe hybridization sites, meaning that FISH karyotyping needs to be supported for the identification of non-intact chromosomes by a GISH-based analysis. For example, the sites of pSc119.2 hybridization are concentrated close to the telomeres of most chromosome arms, making it difficult to differentiate between chromosomes 1Ug, 2Ug, 3Mg, and 4Mg [10, 12]. Here, the objective was to develop a FISH assay able to unequivocally recognize each of the 14 Ae. geniculata chromosomes, and to use this assay to characterize a number of derivatives of an Ae. geniculata × wheat wide cross.
机译：Backgroundaegilops geniculata roth（syn。ae。ovata l，2n？=Δ4x？=Δ28，基因组公式Ugmg）表示与面包小麦改善相关的潜在遗传变异的源。该物种被认为代表二倍体AE之间的天然同种异体四倍体。 Umbellulata Zhuk。 （U基因组载体）和AE。 Comosa Sibth。 et sm。 （M个基因组载体）[1]。赋予各种疾病耐药的许多基因已从该物种转移到面包小麦中，特别是YR40（抗条纹锈病），LR57（叶锈）[2]，SR53（Step Rust）[3]和PM29（粉末状霉菌）[4,5]。 ae的一些accessions。 Geniculata显示出高水平的水使用效率[6]，并且物种总体上表现出比任何相关的物种AE的水分应激较高的耐受性。 Markgrafii（Gruiter）K. Hammer，AE。朗根斯施韦夫夫。 ＆Muschl。，AE。 Searsii Feldman et Kislev Ex K. Hammer或Ae。 Spottoides Tausch [7]。它还携带在编码胚乳蛋白的基因上的等位基因预测，预测，提高了面包小麦的最终使用质量[8,9]。原位杂交（GISH）是一种非常成功的技术，用于区分属于的染色体在Triticeae物种中表示的各种基因组，而原位杂交（鱼）的荧光倾向于用于鉴定单个染色体。一种鱼类的AE核型。已经建立了Geniculata，采用探针组合PSC119.2，AFA家族重复，PAS1和PTA71 [10,11,12]。但是，AE的一些部分。 Geniculata Genome缺乏任何探针杂交位点，这意味着需要通过基于Gish的分析来支持鱼核型来鉴定非完整染色体。例如，PSC119.2杂交的位点浓缩靠近大多数染色体臂的端粒，使得染色体1ug，2ug，3mg和4mg [10,12]难以区分。这里，该目的是开发一种能够明确地识别14 AE中的鱼类的鱼类测定。 Geniculata染色体，并使用该测定表征AE的许多衍生物。 Geniculata×小麦宽十字架。

著录项

相似文献

  • 外文文献
  • 中文文献
  • 专利
获取原文

客服邮箱：kefu@zhangqiaokeyan.com

京公网安备：11010802029741号 ICP备案号：京ICP备15016152号-6 六维联合信息科技 (北京) 有限公司©版权所有

  • 客服微信

  • 服务号