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首页> 外文期刊>mSphere >Streptococcus gordonii Type I Lipoteichoic Acid Contributes to Surface Protein Biogenesis
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Streptococcus gordonii Type I Lipoteichoic Acid Contributes to Surface Protein Biogenesis

机译：链球菌型I脂素酸型含有表面蛋白质生物发生

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Lipoteichoic acid (LTA) is an abundant polymer of the Gram-positive bacterial cell envelope and is essential for many species. Whereas the exact function of LTA has not been elucidated, loss of LTA in some species affects hydrophobicity, biofilm formation, and cell division. Using a viable LTA-deficient strain of the human oral commensal Streptococcus gordonii , we demonstrated that LTA plays an important role in surface protein presentation. Cell wall fractions derived from the wild-type and LTA-deficient strains of S. gordonii were analyzed using label-free mass spectroscopy. Comparisons showed that the abundances of many proteins differed, including (i) SspA, SspB, and S. gordonii 0707 (SGO_0707) (biofilm formation); (ii) FtsE (cell division); (iii) Pbp1a and Pbp2a (cell wall biosynthesis and remodeling); and (iv) DegP (envelope stress response). These changes in cell surface protein presentation appear to explain our observations of altered cell envelope homeostasis, biofilm formation, and adhesion to eukaryotic cells, without affecting binding and coaggregation with other bacterial species, and provide insight into the phenotypes revealed by the loss of LTA in other species of Gram-positive bacteria. We also characterized the chemical structure of the LTA expressed by S. gordonii . Similarly to Streptococcus suis , S. gordonii produced a complex type I LTA, decorated with multiple d -alanylations and glycosylations. Hence, the S. gordonii LTA appears to orchestrate expression and presentation of cell surface-associated proteins and functions. IMPORTANCE Discovered over a half-century ago, lipoteichoic acid (LTA) is an abundant polymer found on the surface of Gram-positive bacteria. Although LTA is essential for the survival of many Gram-positive species, knowledge of how LTA contributes to bacterial physiology has remained elusive. Recently, LTA-deficient strains have been generated in some Gram-positive species, including the human oral commensal Streptococcus gordonii . The significance of our research is that we utilized an LTA-deficient strain of S. gordonii to address why LTA is physiologically important to Gram-positive bacteria. We demonstrate that in S. gordonii , LTA plays an important role in the presentation of many cell surface-associated proteins, contributing to cell envelope homeostasis, cell-to-cell interactions in biofilms, and adhesion to eukaryotic cells. These data may broadly reflect a physiological role of LTA in Gram-positive bacteria.
机译：Lipoteichic酸（LTA）是革兰氏阳性细菌细胞包膜的丰富聚合物，对于许多物种至关重要。虽然尚未阐明LTA的确切功能，但某些物种中LTA的丧失会影响疏水性，生物膜形成和细胞分裂。利用可行的LTA缺乏的人口青少年链球菌的缺乏菌株，我们证明LTA在表面蛋白呈现中起重要作用。使用无标记的质谱分析源自野生型和LTA缺乏的S.GOLDONII菌株的细胞壁级分。比较表明，许多蛋白质的丰富不同，包括（i）sspa，sspb和s. gordonii 0707（sgo_0707）（生物膜形成）; （ii）FTSE（细胞划分）; （iii）PBP1A和PBP2A（细胞壁生物合成和重塑）; （IV）DEGP（信封应力响应）。细胞表面蛋白介绍的这些变化似乎解释了我们对对真核细胞的改变的细胞包膜稳态，生物膜形成和粘附性的观察，而不会影响与其他细菌种类的结合和共ggration，并提供对LTA丧失表现出来的表型的洞察力其他种类的革兰氏阳性细菌。我们还表征了由S. Gordonii表达的LTA的化学结构。类似于链球菌Suis，S.Gordonii产生了一种复杂的I型LTA，用多个-alAnylation和糖基化装饰。因此，S.Gordonii LTA似乎衡了细胞表面相关蛋白和功能的表达和呈递。在半个世纪前发现的重要性，Lipoteichoic酸（LTA）是一种在革兰氏阳性细菌表面上发现的丰富聚合物。尽管LTA对于许多革兰氏阳性物种的生存至关重要，但了解LTA如何对细菌生理有助于难以捉摸。最近，已经在一些革兰氏阳性物种中产生了LTA缺陷的菌株，包括人口腔共生链球菌Gordonii。我们的研究的意义是，我们利用LTA缺乏的S. Gordonii菌株来解决原因LTA对革兰氏阳性细菌的生理学。我们证明，在S.Gordonii中，LTA在许多细胞表面相关蛋白质的呈现中起重要作用，有助于细胞包膜稳态，生物膜中的细胞对细胞相互作用，以及对真核细胞的粘附性。这些数据可以广泛地反映LTA在革兰氏阳性细菌中的生理作用。

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