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首页> 外文期刊>Nepal Journal of Biotechnology >Phytochemical Evaluation, Antioxidant and Antimicrobial Activities of Various Extracts from Leaves and Stems of Bryophyllum pinnatum
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Phytochemical Evaluation, Antioxidant and Antimicrobial Activities of Various Extracts from Leaves and Stems of Bryophyllum pinnatum

机译：来自叶片和茎秆茎和茎的植物化学评价，抗氧化剂和抗菌活性叶片和茎秆茎

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Antioxidant and antimicrobial activities of different extracts (methanol and ethyl acetate) of leaf and stem ofBryophyllum pinnatum were studied. The screening for the secondary metabolites was carried out using thestandard methods. The antioxidant capacities of the different extracts were assessed using DPPH (2,2-diphenyl-1-picrylhydrazyl) radicals and Ferric reducing antioxidant power (FRAP) while the antimicrobial activity ofthe extracts obtained were screened against Gram-positive, Gram-negative bacteria and fungi (Staphylococcusaureus, Escherichia coli, Pseudomonas aeruginosa, Salmonella spp., Vibrio cholerae, Candida albicans and Aspergillusniger) using Agar well diffusion method. Both extracts obtained from leaf and stem of B. pinnatum containedmost of the phytochemical compounds tested for. However, anthocyanins and anthraquinone were notdetected in leaf extracts while coumarin was absent in stem extracts. Quantification of bioactive compoundsshowed that both extracts contained the highest concentration of polyphenols (34.49 ±0.47 mg GAE/g and 32.32±1.2 mg GAE/g for methanol leaf and stem extracts respectively) while the least concentration was recordedfor alkaloids (0.03±0.02 mg/g for methanol stem extract). Results revealed that the extracts showed dosedependent scavenging of DPPH as well as the ability of the extracts to reduce FeCl3 solution, with methanolextracts exhibiting the highest scavenging and reducing capacity. However the leaves of B. pinnatum hadgreater antioxidant activity than the stem by DPPH and ferric reducing assays, with IC50 values ranging from3.147μg/ml to 3.80μg/ml for DPPH and 331.9 - 451 μg/ml for FRAP assays. The antimicrobial activity ofvarious solvent extracts of leaf and stem reveal that microorganisms exhibited different sensitivities towardsthese extracts in a dose-dependent manner. Methanol leaf extract showed no activity against E. coli while P.aeruginosa was insensitive to ethyl acetate leaf extract. For stem extracts, A. niger, V. cholerae and P. aeruginosawere resistant to methanol extract while A. niger, Salmonella spp. and P. aeruginosa was resistant towards ethylacetate stem extract. The results obtained in this study showed that B. pinnatum is a reservoir of bioactivecompounds and both extracts exhibited significant antimicrobial and antioxidant activity.
机译：研究了不同提取物（甲醇和乙酸乙酯）的抗氧化剂和抗微生物活性，叶片和茎的茎和茎。使用鉴定方法进行次级代谢物的筛选。使用DPPH（2,2-二苯基-1-富铬酰基）自由基评估不同提取物的抗氧化能力，并且还原抗氧化能力（FRAP），而获得的提取物的抗微生物活性被筛选抗革兰氏阳性，革兰氏阴性细菌和真菌（葡萄球菌，大肠杆菌，假单胞菌Aeruginosa，Salmonella SPP，SPP，霍乱，念珠菌，念珠菌和Aspergillusniger）使用琼脂井扩散方法。两种从叶片和B的茎中获得的摘录。含有植物化合物的茎状物测试。然而，在叶子提取物中缩短了花青素和蒽醌，而香豆素在茎提取物中不存在。其含有生物活性化合物的定量，即两种提取物含有最高浓度的多酚（34.49±0.47mg，分别为甲醇叶和干提取物32.32±1.2mg gae / g），而生物碱的最低浓度被记录（0.03±0.02mg / g用于甲醇干萃取物）。结果表明，提取物显示DPPH的依赖性清除，以及提取物减少FECL3溶液的能力，甲醇萃取物表现出最高的清除和降低能力。然而，B.Pinnatum Hadgreater抗氧化活性的抗氧化活性比DPPH和铁还原测定，IC 50值范围为3.147μg/ ml至3.80μg/ ml，用于DPPH和331.9-451μg/ ml，用于FRAP测定。叶子和茎的抗微生物活性溶剂萃取物揭示微生物以剂量依赖性方式表现出不同的敏感性。甲醇叶片提取物在P.Aeruginosa对乙酸乙酯叶提取物不敏感的情况下，没有针对大肠杆菌的活性。对于茎萃取物，A.Niger，V.霍乱和铜绿假疗法抵抗甲醇提取物，而A.Niger，Salmonella SPP。和P.铜绿假单胞菌对乙酸乙酯干提取物耐药。本研究中获得的结果表明，B.Pinnatum是生物术中化合物的储层，并且两种提取物都表现出显着的抗微生物和抗氧化活性。

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