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首页> 外文期刊>ScientificWorldJournal >A Novel HPLC Method for the Concurrent Analysis and Quantitation of Seven Water-Soluble Vitamins in Biological Fluids (Plasma and Urine): A Validation Study and Application
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A Novel HPLC Method for the Concurrent Analysis and Quantitation of Seven Water-Soluble Vitamins in Biological Fluids (Plasma and Urine): A Validation Study and Application

机译：一种新型HPLC方法，用于生物液中七种水溶性维生素的同步分析和定量（血浆和尿液）：验证研究与应用

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摘要

An HPLC method was developed and validated for the concurrent detection and quantitation of seven water-soluble vitamins (C, B1, B2, B5, B6, B9, B12) in biological matrices (plasma and urine). Separation was achieved at 30°C on a reversed-phase C18-A column using combined isocratic and linear gradient elution with a mobile phase consisting of 0.01% TFA aqueous and 100% methanol. Total run time was 35 minutes. Detection was performed with diode array set at 280 nm. Each vitamin was quantitatively determined at its maximum wavelength. Spectral comparison was used for peak identification in real samples (24 plasma and urine samples from abstinent alcohol-dependent males). Interday and intraday precision were <4% and <7%, respectively, for all vitamins. Recovery percentages ranged from 93% to 100%.
机译：开发了HPLC方法并验证了生物基质（血浆和尿液）的七种水溶性维生素（C，B1，B2，B5，B6，B9，B12）的七种水溶性维生素（C，B1，B2，B5，B6，B9，B12）的同步检测和定量。使用具有0.01％TFA水溶液和100％甲醇组成的流动相，在逆转相C18-A柱上在30℃下在30℃下进行分离。总运行时间为35分钟。使用在280nm处设置的二极管阵列进行检测。每种维生素在其最大波长上定量地确定。光谱比较用于真实样品中的峰值鉴定（来自戒备酗酒的雄性的24个血浆和尿液）。对于所有维生素，间隔前和间间精度分别为<4％和<7％。恢复百分比范围为93％至100％。

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