...
  • 主题
高级搜索  >
历史搜索 清空历史
首页> 外文期刊>Stem cells international >Therapeutic Effects of Human Urine-Derived Stem Cells in a Rat Model of Cisplatin-Induced Acute Kidney Injury In Vivo and In Vitro
【24h】

Therapeutic Effects of Human Urine-Derived Stem Cells in a Rat Model of Cisplatin-Induced Acute Kidney Injury In Vivo and In Vitro

机译：中铂诱导的急性肾损伤大鼠模型中人类尿衍生干细胞的治疗效果

获取原文
           
页面导航
  • 摘要
  • 著录项
  • 相似文献
  • 相关主题

摘要

Acute kidney injury (AKI) is an extremely dangerous clinical syndrome with high morbidity and mortality. Stem cell-based therapies have shown great promise for AKI treatment. Urine-derived stem cells (USCs) are a novel cell source in tissue engineering and cell therapy which provide advantages of simple, noninvasive, and low-cost harvest methods, efficient proliferation, and multi-differentiation potential. Here, we described the therapeutic effects of USCs in a rat model of cisplatin-induced AKI as a novel therapy. In vivo, the intravenous administration of USCs alleviated the renal functional damage in AKI rats, for the levels of blood urea nitrogen (BUN) and serum creatinine (SCr) were significantly decreased. The USCs-treated group also exhibited improved histological and ultrastructural changes, promoted proliferation, and inhibited apoptosis in renal tissues. After the USC therapy, the expression levels of proinflammatory cytokines (TNF-α and IL-6) and apoptosis-related proteins (BAX and cleaved caspase-3) were downregulated. In addition, the presence of a few GFP-labeled USCs was confirmed in rat renal tissues. In vitro, rat tubular epithelial (NRK-52E) cells were incubated with cisplatin to induce cell damage and then cocultured with USCs. After coculture with USCs, the cisplatin-induced NRK-52E cells showed higher cell viability and a lower apoptosis ratio than those of the control group, and cell cycle arrest was improved. In conclusion, our results demonstrated that USC therapy significantly improved the renal function and histological damage, inhibited the inflammation and apoptosis processes in the kidney, and promoted tubular epithelial proliferation. Our study exhibited the potential of USCs in the treatment of AKI, representing a new clinical therapeutic strategy.
机译：急性肾脏损伤（AKI）是一种极其危险的临床综合征，发病率高，死亡率高。基于干细胞的疗法对AKI治疗表示了很好的承诺。尿液衍生的干细胞（USCs）是组织工程和细胞疗法中的新细胞来源，其具有简单，无侵入性和低成本的收获方法，高效增殖和多分化潜力的优点。在这里，我们描述了USCS在顺铂诱导的AKI大鼠模型中的治疗效果作为新疗法。体内，USCs的静脉内施用减轻了AKI大鼠的肾功能损伤，对于血尿尿素氮（BUN）和血清肌酐（SCR）的水平显着降低。 USCS治疗组还表现出改善的组织学和超微结构变化，促进增殖，抑制肾组织的细胞凋亡。 USC治疗后，下调促炎细胞因子（TNF-α和IL-6）和凋亡相关蛋白（Bax和Celleaved Caspase-3）的表达水平。此外，在大鼠肾组织中证实了几种GFP标记的USCs的存在。体外，将大鼠管状上皮（NRK-52E）细胞与顺铂一起孵育，以诱导细胞损伤，然后用USCS与USC一起获得。通过USCS与USC进行共核，顺铂诱导的NRK-52E细胞显示出更高的细胞活力和比对照组的凋亡比较低，细胞周期停留得到改善。总之，我们的结果表明，USC治疗显着提高了肾功能和组织学损伤，抑制肾脏中的炎症和凋亡过程，促进管状上皮增殖。我们的研究表明USCS在治疗AKI中的潜力，代表了一种新的临床治疗策略。

著录项

相似文献

  • 外文文献
  • 中文文献
  • 专利
获取原文

客服邮箱：kefu@zhangqiaokeyan.com

京公网安备：11010802029741号 ICP备案号：京ICP备15016152号-6 六维联合信息科技 (北京) 有限公司©版权所有

  • 客服微信

  • 服务号