Protein isolation and identification of trichosanthes kirilowii seed from different places of production

Jin Xie; Xiaoqi Zhu; Rong Song; Siwen Peng; Yangning Huang

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Protein isolation and identification of trichosanthes kirilowii seed from different places of production

机译：不同生产区的蛋白质分离和鉴定基洛韦氏种子

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In this paper, 12 samples of Trichosanthes kirilowii seed from different places of production were studied. The total proteins of the 12 samples were extracted by Tris-phenol method. Twelve samples of proteins were isolated by SDS-PAGE. SDS-PAGE results showed that the protein bands in sample No.8 are different from those of other samples. And those protein bands were identified by mass spectrometry. At least 10 differentially expressed proteins were identified, including rRNA N-glycosidase, Acetyl-CoA acyltransferase, Citrate synthase, Phosphoglycerate kinase, Isocitrate dehydrogenase, Fructose-bisphosphate aldolase, Ferritin, Urease, Hhydroxyacyl-ACP-dehydratase, Glutathione peroxidase. From the result of protein identification, it can be inferred that the seed of No. 8 sample was superior to other samples during germination.

机译：在本文中，研究了来自不同生产地点的12种richosanthes kirilowii种子。通过Tris-苯酚法提取12样品的总蛋白质。通过SDS-PAGE分离12种蛋白质样品。 SDS-PAGE结果表明，样品中的蛋白质条带与其他样品的蛋白质带不同。并通过质谱法鉴定这些蛋白质条带。鉴定了至少10个差异表达的蛋白质，包括rRNA n-糖苷酶，乙酰基 - 酰基酰基转移酶，柠檬酸合酶，磷酸糖激酶，异柠檬酸脱氢酶，果糖 - 双磷酸醛糖酶，铁蛋白，脲酶，羟基苯基-ACP-脱水酶，谷胱甘肽过氧化物酶。从蛋白质鉴定的结果，可以推断出8号样品的种子在萌发期间优于其他样品。

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《Pakistan journal of botany》 |2020年第1期|共6页
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Jin Xie; Xiaoqi Zhu; Rong Song; Siwen Peng; Yangning Huang;
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Trichosanthes kirilowii seedProtienIsolation and Identification.;

机译：Trichosanthes Kirilowii种子保护和鉴定。;

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Protein isolation and identification of trichosanthes kirilowii seed from different places of production

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