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首页> 外文期刊>Parasites Vectors >DNA barcoding reveals both known and novel taxa in the Albitarsis Group (Anopheles: Nyssorhynchus) of Neotropical malaria vectors
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DNA barcoding reveals both known and novel taxa in the Albitarsis Group (Anopheles: Nyssorhynchus) of Neotropical malaria vectors

机译：DNA条形码揭示了肌肤疟疾载体的Albitarsis组（Anopheles：Nyssorhynchus）中已知和新的分类群

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Background Mosquitoes belonging to the Albitarsis Group (Anopheles: Nyssorhynchus) are of importance as malaria vectors across the Neotropics. The Group currently comprises six known species, and recent studies have indicated further hidden biodiversity within the Group. DNA barcoding has been proposed as a highly useful tool for species recognition, although its discriminatory utility has not been verified in closely related taxa across a wide geographic distribution. Methods DNA barcodes (658 bp of the mtDNA Cytochrome c Oxidase - COI) were generated for 565 An. albitarsis s.l. collected in Argentina, Brazil, Colombia, Paraguay, Trinidad and Venezuela over the past twenty years, including specimens from type series and type localities. Here we test the utility of currently advocated barcoding methodologies, including the Kimura-two-parameter distance model (K2P) and Neighbor-joining analysis (NJ), for determining species delineation within mosquitoes of the Neotropical Albitarsis Group of malaria vectors (Anopheles: Nyssorhynchus), and compare results with Bayesian analysis. Results Species delineation through barcoding analysis and Bayesian phylogenetic analysis, fully concur. Analysis of 565 sequences (302 unique haplotypes) resolved nine NJ tree clusters, with less than 2% intra-node variation. Mean intra-specific variation (K2P) was 0.009 (range 0.002 - 0.014), whereas mean inter-specific divergence were several-fold higher at 0.041 (0.020 - 0.056), supporting the reported "barcoding gap". These results show full support for separate species status of the six known species in the Albitarsis Group (An. albitarsis s.s., An. albitarsis F, An. deaneorum, An. janconnae, An. marajoara and An. oryzalimnetes), and also support species level status for two previously detected lineages - An. albitarsis G & An. albitarsis I (designated herein). In addition, we highlight the presence of a unique mitochondrial lineage close to An. deaneorum and An. marajoara (An. albitarsis H) from Rond?nia and Mato Grosso in southwestern Brazil. Further integrated studies are required to confirm the status of this lineage. Conclusions DNA barcoding provides a reliable means of identifying both known and undiscovered biodiversity within the closely related taxa of the Albitarsis Group. We advocate its usage in future studies to elucidate the vector competence and respective distributions of all eight species in the Albitarsis Group and the novel mitochondrial lineage (An. albitarsis H) recovered in this study.
机译：背景蚊子属于Albitarsis组（anopheles：nyssorhynchus）对新生物的疟疾载体具有重要性。该组目前包含六种已知的物种，最近的研究表明该组内进一步隐藏的生物多样性。 DNA条形码已被提出为物种识别的高度有用的工具，尽管其歧视性效用尚未在广泛的地理分布上密切相关。方法产生DNA条形码（MTDNA细胞色素C氧化酶 - COI的658BP），565例。 Albitarsis S.L.在过去的二十年中，在阿根廷，巴西，哥伦比亚，巴拉圭，特立尼达和委内瑞拉收集，包括来自系列系列和类型的标本。在这里，我们测试目前主张的条形码方法的效用，包括Kimura-Tway-Perizess距离模型（K2P）和邻接分析（NJ），用于在疟疾载体的新疗法Albitarsis组的蚊子中确定物种描绘（anopheles：nyssorhynchus ），并将结果与​​贝叶斯分析进行比较。结果通过条形码分析和贝叶斯系统发育分析划分物种划分，完全同样。分析565个序列（302个独特的单倍型）分辨九个NJ树簇，内部节点内的少于2％。平均特定的变异（K2P）为0.009（范围0.002-0.014），而平均特异性发散在0.041（0.020-0.056）时几倍较高，支持报道的“条形码差距”。这些结果表明Albitarsis组中的六种已知物种的单独物种状态（Albitarsis ss，A.Anbitarsis F，A.Deaneorum，A.Janconnae，A.AnyzalimNetes），以及oryzalimnetes的六个。两个先前检测到的谱系的级别状态 - AN。 albitarsis g＆an。 Albitarsis I（在此指定）。此外，我们突出了靠近A的独特线粒体谱系的存在。 Deaneorum和An。 Marajoara（Albitarsis H）来自rund？Nia和巴西西南部的Mato Grosso。需要进一步的综合研究来确认这种谱系的地位。结论DNA条形码提供了一种可靠的方法，可在Albitarsis集团的密切相关征草中识别已知和未被发现的生物多样性。我们倡导其在未来的研究中的使用，以阐明在本研究中恢复的Albitarsis组和新型线粒体谱系（Albitarsis H）中所有八种物种的传染媒介能力和各自的分布。

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