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Identification and characterization of six novel tetraspanins from Schistosoma japonicum

机译：日本血吸虫血吸虫六种新型四苯磺辛鉴定及表征

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Background Tetraspanins (TSPs), also known as members of the trans-membrane 4 super-family (TM4SF), comprise an assemblage of surface antigens reported in eukaryotic organisms. In the work presented here, six novel TSP proteins from the human blood fluke Schistosoma japonicum (S. japonicum) were produced and analyzed through a combination of bioinformatics and experimental approaches. Results Six novel TSP proteins of Schistosoma japonicum (designated as Sj-TSP-#1~6) contained four trans-membrane regions and one large extracellular loop (LEL) with a conserved CCG motif. Size of the proteins varied from 227 to 291 amino acid residues. All the six proteins were produced in E.coli and immune sera to each protein were prepared. Analysis of transcription profiles of the proteins by RT-PCR showed that Sj-TSP-#4 was transcribed only in the egg stage while transcription of the Sj-TSP-#2 was detected in female worms but not in males. The similar results were obtained by Western blot. Immunolocalization of the TSP proteins by immunofluorescence assay showed that the Sj-TSP-#2, Sj-TSP-#5 and Sj-TSP-#6 were located in the tegument of worms. Conclusions This study provided six novel TSP members of S. japonicum including their sequences and recombinant proteins. Availability of the novel proteins and information on their expression profile and location provided a basis for further investigation of the TSP proteins for their biological functions and as vaccine candidates.
机译：背景技术Tetraspanins（TSP），也称为跨膜4超级家庭（TM4SF）的构件，包括在真核生物中报告的表面抗原的组合。在此处提供的工作中，通过生物信息学和实验方法的组合产生并分析来自人血氟氏血吸虫的六种新的TSP蛋白质。结果血吸虫血吸虫的六种新型TSP蛋白（指定为SJ-TSP-＃1〜6）含有四个跨膜区和一个具有保守CCG基序的一个大细胞外环（LEL）。蛋白质的大小从227变化到291〜291个氨基酸残基。所有六种蛋白质在大肠杆菌中制备，并制备每种蛋白质的免疫血清。通过RT-PCR分析蛋白质的转录谱表明，仅在鸡蛋阶段转录SJ-TSP-＃4，同时在雌性蠕虫中检测到SJ TSP-＃2的转录，但不在雄性中。通过Western印迹获得了类似的结果。通过免疫荧光测定的TSP蛋白的免疫循环化表明，SJ-TSP-＃2，SJ-TSP-＃5和SJ-TSP-＃6位于蠕虫的Teguments中。结论本研究提供了六个新的TSP成员，包括它们的序列和重组蛋白。新型蛋白质的可用性和关于它们的表达谱和位置的信息提供了一种进一步研究其生物学功能和疫苗候选的TSP蛋白的基础。

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