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首页> 外文期刊>Plant Biotechnology Journal >Transcriptome analysis reveals a comprehensive insect resistance response mechanism in cotton to infestation by the phloem feeding insect Bemisia tabaci (whitefly)
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Transcriptome analysis reveals a comprehensive insect resistance response mechanism in cotton to infestation by the phloem feeding insect Bemisia tabaci (whitefly)

机译：转录组分析揭示了棉花的综合性抗虫反应机制，由植物饲喂昆虫菌塔布（粉虱）的侵袭

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Summary The whitefly ( Bemisia tabaci ) causes tremendous damage to cotton production worldwide. However, very limited information is available about how plants perceive and defend themselves from this destructive pest. In this study, the transcriptomic differences between two cotton cultivars that exhibit either strong resistance (HR) or sensitivity (ZS) to whitefly were compared at different time points (0, 12, 24 and 48?h after infection) using RNA-Seq. Approximately one billion paired-end reads were obtained by Illumina sequencing technology. Gene ontology and KEGG pathway analysis indicated that the cotton transcriptional response to whitefly infestation involves genes encoding protein kinases, transcription factors, metabolite synthesis, and phytohormone signalling. Furthermore, a weighted gene co-expression network constructed from RNA-Seq datasets showed that WRKY40 and copper transport protein are hub genes that may regulate cotton defenses to whitefly infestation. Silencing GhMPK3 by virus-induced gene silencing (VIGS) resulted in suppression of the MPK-WRKY-JA and ET pathways and lead to enhanced whitefly susceptibility, suggesting that the candidate insect resistant genes identified in this RNA-Seq analysis are credible and offer significant utility. Taken together, this study provides comprehensive insights into the cotton defense system to whitefly infestation and has identified several candidate genes for control of phloem-feeding pests.
机译：发明内容粉虱（Bemisia Tabaci）对全球棉花生产造成巨大损坏。但是，有限的信息是如何从这种破坏性害虫感知和保护自己的有限的信息。在这项研究中，在使用RNA-SEQ的不同时间点（0,12,20,24和48℃）比较了两种棉质品种之间的转录组差异，其表现出强烈的抗性（HR）或敏感性（ZS）与粉虱进行比较。通过Illumina测序技术获得了大约十亿个配对读数。基因本体和Kegg途径分析表明，棉花转录对粉虱感染的反应涉及编码蛋白激酶，转录因子，代谢物合成和植物激素信号传导的基因。此外，由RNA-SEQ数据集构成的加权基因共表达网络显示WRKY40和铜转运蛋白是可以调节棉花防御的轮毂基因对粉虱感染。通过病毒诱导的基因沉默（Vigs）沉默GHMPK3导致抑制MPK-WRKY-JA和ET途径，并导致粉虱敏感性增强，表明该RNA-SEQ分析中鉴定的候选抗虫基因是可信的，并提供重要的公用事业。这项研究在一起，为棉花系统提供了全面的见解，以粉刷粉虱侵袭，并确定了几种候选基因，用于控制饲料饲料的害虫。

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