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The Arabidopsis thaliana N-recognin E3 ligase PROTEOLYSIS1 influences the immune response

机译：拟南芥N-Impical e3连接酶蛋白水溶液影响免疫应答

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N-degron pathways of ubiquitin-mediated proteolysis (formerly known as the N-end rule pathway) control the stability of substrate proteins dependent on the amino-terminal (Nt) residue. Unlike yeast or mammalian N-recognin E3 ligases, which each recognize several different classes of Nt residues, in Arabidopsis thaliana, N-recognin functions of different N-degron pathways are carried out independently by PROTEOLYSIS (PRT)1, PRT6, and other unknown proteins. PRT1 recognizes type 2 aromatic Nt-destabilizing residues and PRT6 recognizes type 1 basic residues. These two N-recognin functions diverged as separate proteins early in the evolution of plants, before the conquest of the land. We demonstrate that loss of PRT1 function promotes the plant immune system, as mutant prt1-1 plants showed greater apoplastic resistance than WT to infection by the bacterial hemi-biotroph Pseudomonas syringae pv tomato (Pst) DC3000. Quantitative proteomics revealed increased accumulation of proteins associated with specific components of plant defense in the prt1-1 mutant, concomitant with increased accumulation of salicylic acid. The effects of the prt1 mutation were additional to known effects of prt6 in influencing the immune system, in particular, an observed over-accumulation of pipecolic acid (Pip) in the double-mutant prt1-1 prt6-1. These results demonstrate a potential role for PRT1 in controlling aspects of the plant immune system and suggest that PRT1 limits the onset of the defense response via degradation of substrates with type 2 Nt-destabilizing residues.? 2019 The Authors. Plant Direct published by American Society of Plant Biologists, Society for Experimental Biology and John Wiley & Sons Ltd.
机译：泛素介导的蛋白水解的N-血管途径（以前称为N末端规则途径）控制依赖于氨基末端（NT）残基的底物蛋白的稳定性。与酵母或哺乳动物N-IMPACTIN E3连接酶不同，其每种识别出几种不同类别的NT残基，在拟南芥中，不同N-DIPRON途径的N-Impication函数由蛋白水解（PRT）1，PRT6和其他未知而独立进行。蛋白质。 PRT1识别2型芳香族NT - 稳定残留物，PRT6识别1型基本残留物。这两种N-Expectin函数随着植物演变的早期分歧，在征服土地之前。我们证明PRT1功能的丧失促进了植物免疫系统，因为突变体PRT1-1植物显示出比对细菌Hemi-Biotroph假蛋白皂苷PV番茄（PST）DC3000的影响更大的妊娠抗性。定量蛋白质组学显示出PRT1-1突变体中植物防御特异性组分相关的蛋白质积累的增加，伴随着水杨酸的增加。 PRT1突变的效果是Prt6在影响免疫系统中的已知效果，特别是在双突变体PRT1-1 PRT6-1中观察到的吡酸（PIP）的过度积累。这些结果表明PRT1在控制植物免疫系统的方面时对PRT1的潜在作用，并表明PRT1通过含有2型NT - 稳定残留物的底物的降解限制防御响应的发作。？ 2019年的作者。植物直接发表美国植物生物学家，实验生物学学会和约翰瓦里和儿子有限公司

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