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首页> 外文期刊>PLoS Genetics >Cell cycle transcriptomics of Capsaspora provides insights into the evolution of cyclin-CDK machinery
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Cell cycle transcriptomics of Capsaspora provides insights into the evolution of cyclin-CDK machinery

机译：<斜视> CapsaSpora 的细胞周期转录组科为Cyclin-CDK机械的演变提供了见解

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Progression through the cell cycle in eukaryotes is regulated on multiple levels. The main driver of the cell cycle progression is the periodic activity of cyclin-dependent kinase (CDK) complexes. In parallel, transcription during the cell cycle is regulated by a transcriptional program that ensures the just-in-time gene expression. Many core cell cycle regulators are widely conserved in eukaryotes, among them cyclins and CDKs; however, periodic transcriptional programs are divergent between distantly related species. In addition, many otherwise conserved cell cycle regulators have been lost and independently evolved in yeast, a widely used model organism for cell cycle research. For a better understanding of the evolution of the cell cycle regulation in opisthokonts, we investigated the transcriptional program during the cell cycle of the filasterean Capsaspora owczarzaki , a unicellular species closely related to animals. We developed a protocol for cell cycle synchronization in Capsaspora cultures and assessed gene expression over time across the entire cell cycle. We identified a set of 801 periodic genes that grouped into five clusters of expression over time. Comparison with datasets from other eukaryotes revealed that the periodic transcriptional program of Capsaspora is most similar to that of animal cells. We found that orthologues of cyclin A, B and E are expressed at the same cell cycle stages as in human cells and in the same temporal order. However, in contrast to human cells where these cyclins interact with multiple CDKs, Capsaspora cyclins likely interact with a single ancestral CDK1-3. Thus, the Capsaspora cyclin-CDK system could represent an intermediate state in the evolution of animal-like cyclin-CDK regulation. Overall, our results demonstrate that Capsaspora could be a useful unicellular model system for animal cell cycle regulation.
机译：通过细胞周期中的进展在多个层面上受到调节。细胞周期进展的主要驱动器是细胞周期蛋白依赖性激酶（CDK）复合物的周期性活性。平行，细胞周期中的转录由可确保立即基因表达的转录程序调节。许多核心细胞周期调节器在真核节中广泛保守，其中包括细胞周期和CDKS;然而，定期转录方案在远处相关物种之间存在不同。此外，许多否则保护的细胞周期调节剂已经丢失并在酵母中独立地演变，是一种用于细胞周期研究的广泛使用的模型生物体。为了更好地了解Opisthokonts中细胞周期调节的演变，我们在Filsterean Capspora Owczarzaki的细胞周期中调查了转录程序，这是一种与动物密切相关的单细胞物种。我们开发了一种用于Capsaspora培养物中细胞周期同步的方案，并随着时间的推移在整个细胞周期中评估基因表达。我们鉴定了一组801个定期基因，随着时间的推移分为五个表达簇。与其他真核仪的数据集的比较显示，辣椒孢子的周期性转录程序与动物细胞最相似。我们发现细胞周期蛋白A，B和E的矫形器在与人体细胞中的相同细胞周期阶段和相同的时间顺序中表达。然而，与人体细胞相反，这些细胞素与多个CDK相互作用，Capsaspora Cyclins可能与单一祖先CDK1-3相互作用。因此，Capsaspora cyclin-CDK系统可以代表动物样细胞周期蛋白CDK调节的演变中的中间状态。总体而言，我们的结果表明，CapsaSpora可以是用于动物细胞周期调节的有用的单细胞模型系统。

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