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首页> 外文期刊>PLoS Genetics >Sycp2 is essential for synaptonemal complex assembly, early meiotic recombination and homologous pairing in zebrafish spermatocytes
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Sycp2 is essential for synaptonemal complex assembly, early meiotic recombination and homologous pairing in zebrafish spermatocytes

机译：Sycp2对于Synaponemal复合物组装，早期减数分裂重组和在斑马鱼精胶质细胞中的同源配对至关重要

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Meiotic recombination is essential for faithful segregation of homologous chromosomes during gametogenesis. The progression of recombination is associated with dynamic changes in meiotic chromatin structures. However, whether Sycp2, a key structural component of meiotic chromatin, is required for the initiation of meiotic recombination is still unclear in vertebrates. Here, we describe that Sycp2 is required for assembly of the synaptonemal complex and early meiotic events in zebrafish spermatocytes. Our genetic screening by N-ethyl-N-nitrosourea mutagenesis revealed that ietsugu ( its ), a mutant zebrafish line with an aberrant splice site in the sycp2 gene, showed a defect during meiotic prophase I. The its mutation appeared to be a hypomorphic mutation compared to sycp2 knockout mutations generated by TALEN mutagenesis. Taking advantage of these sycp2 hypomorphic and knockout mutant lines, we demonstrated that Sycp2 is required for the assembly of the synaptonemal complex that is initiated in the vicinity of telomeres in wild-type zebrafish spermatocytes. Accordingly, homologous pairing, the foci of the meiotic recombinases Dmc1/Rad51 and RPA, and γH2AX signals were largely diminished in sycp2 knockout spermatocytes. Taken together, our data indicate that Sycp2 plays a critical role in not only the assembly of the synaptonemal complex, but also early meiotic recombination and homologous pairing, in vertebrates.
机译：减数分裂重组对于在配子发生期间对同源染色体的忠实偏析至关重要。重组的进展与减数分裂染色质结构的动态变化有关。然而，无论是减数分子染色质的关键结构组分的SYCP2是否需要在脊椎动物中仍不清楚。在这里，我们描述了Sycp2需要在斑马鱼精胶质细胞中组装Synaponemal综合体和早期减数病。我们的N-乙基-N-亚硝基脲脲脲植物诱变的遗传筛查显示Ietsugu（其），突变体斑马鱼与Sycp2基因中异常剪接位点的突变斑马鱼系，在减数分裂预防期间表现出缺陷。其突变似乎是一种低常突变与TALEN诱变产生的SYCP2敲除突变相比。利用这些Sycp2的低晶和敲除突变线，我们证明了Sycaponemal综合体组装的Sycp2在野生型斑马鱼精胶质细胞的端粒附近启动。因此，在Sycp2敲除精胶质细胞中大大降低了减数分子重组酶DMC1 / RAD51和RPA和γH2AX信号的同源配对。我们的数据表明，Sycp2不仅在蛇形骨骼复合物的组装中起到关键作用，而且在脊椎动物中的早期减数分裂重组和同源配对作用。

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