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首页> 外文期刊>PLoS Genetics >Chromatin accessibility established by Pou5f3, Sox19b and Nanog primes genes for activity during zebrafish genome activation
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Chromatin accessibility established by Pou5f3, Sox19b and Nanog primes genes for activity during zebrafish genome activation

机译：在斑马鱼基因组激活期间，由POU5F3，SOX19B和NANOG引物基因建立的染色质可粘附性

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In many organisms, early embryonic development is driven by maternally provided factors until the controlled onset of transcription during zygotic genome activation. The regulation of chromatin accessibility and its relationship to gene activity during this transition remain poorly understood. Here, we generated chromatin accessibility maps with ATAC-seq from genome activation until the onset of lineage specification. During this period, chromatin accessibility increases at regulatory elements. This increase is independent of RNA polymerase II-mediated transcription, with the exception of the hypertranscribed miR-430 locus. Instead, accessibility often precedes the transcription of associated genes. Loss of the maternal transcription factors Pou5f3, Sox19b, and Nanog, which are known to be required for zebrafish genome activation, results in decreased accessibility at regulatory elements. Importantly, the accessibility of regulatory regions, especially when established by Pou5f3, Sox19b and Nanog, is predictive for future transcription. Our results show that the maternally provided transcription factors Pou5f3, Sox19b, and Nanog open up chromatin and prime genes for activity during zygotic genome activation in zebrafish.
机译：在许多生物中，早期胚胎发育是由母体提供的因素驱动，直到在Zygotic基因组激活期间的转录开始。在这种转变期间的染色质可用性及其与基因活性的关系仍然难以理解。在这里，我们产生了从基因组激活的ATAC-SEQ生成染色质可访问性图，直到谱系规范的开始。在此期间，染色质的可访问性在调节元件增加。这种升高与RNA聚合酶II介导的转录无关，除了过度的miR-430基因座外。相反，可访问性通常在相关基因的转录之前。母体转录因子POU5F3，SOX19B和NANOG的丧失，已知是斑马鱼基因组活化所需的，导致调节元件的可访问性降低。重要的是，监管区域的可访问性，尤其是由POU5F3，SOX19B和NANOG建立时，是未来转录的预测性。我们的研究结果表明，母材提供了转录因子POU5F3，SOX19B和Nanog在斑马鱼中的Zygotic基因组激活期间开放染色质和素基因。

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