Identification of the binding site for plasminogen kringle 5 in the α-chain of fibrin(ogen) D-fragment

L. G. Kapustianenko; T. V. Grinenko; A. V. Rebriev

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Identification of the binding site for plasminogen kringle 5 in the α-chain of fibrin(ogen) D-fragment

机译：纤溶酶原Kringle 5在纤维蛋白（ELIGON）D-碎片中的粒子原Kringle 5的鉴定

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The interaction of the fifth kringle of Glu-plasminogen with fibrin triggers activation and initiation of fibrinolysis, yet the site on fibrin that binds kringle 5 remains unknown. The aim of our work was to determine an amino acid sequence in the D-fragment of fibrin(ogen) molecule, which is complementary to the lysine-binding site (LBS) in kringle 5. We studied the interaction between kringle 5 of plasminogen with polypeptide chains of the D-fragments of fibrin and cyanogen bromide fragments FCB-2 and t-NDSK and showed that kringle 5 bound specifically to α- and γ-chains of the D-fragment and the α-chain of FCB-2. Tryptic peptides of D-fragment α-chain were obtained, separated by their ability to bind with the immobilized kringle 5, and then all studied peptides were characterized by MALDI-TOF analysis. The critical amino acid residues of the α-chain of D-fragment, which provide its interaction with kringle 5, turned out to be α171Arg and/or α176Lys. The binding site of Glu-plasminogen complementary to the LBS of kringle 5 is located within Аα168Ala?183Lys, a sequence in a weakly structured loop between two supercoils in the α-chain of the D-fragment of the fibrin(ogen) molecule.

机译：胶纤溶蛋白的第五kringle与纤维蛋白的相互作用触发纤维蛋白的激活和引发，然而纤维蛋白的位点仍然未知。我们的作品的目的是确定纤维蛋白（ELIGON）分子的D-片段中的氨基酸序列，其与Kringle 5中的赖氨酸结合位点（LBS）互补。我们研究了纤溶酶原的Kringle 5之间的相互作用纤维蛋白和氰基溴化物片段FCB-2和T-NDSK的多肽链和氰基碎片的碎片链条，并显示Kringle 5特异性地结合到D-片段的α-和γ-链和Fcb-2的α-链。获得D-碎片α链的胰蛋白酶肽，通过它们与固定的Kringle 5结合的能力分离，然后通过MALDI-TOF分析表征所有研究的肽。 D-片段的α链的临界氨基酸残基，其与Kringle 5提供其相互作用，结果为α171ARG和/或α176。与Kringle 5的LBS互补的Glu - 纤溶酶原的结合位点位于α168Alaα183ly内，在纤维蛋白（ELIGOL）分子的D-片段的D-片段的α链中的两种超级油之间的弱结构环中。

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《Ukrainian Biochemical Journal》 |2020年第3期|共12页
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L. G. Kapustianenko; T. V. Grinenko; A. V. Rebriev;
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binding sitefibrinogenfibrinolysiskringle 5plasminogenα-chain of fibrin D-fragment;

机译：纤维蛋白D-碎片的粘合SitefibrinogencibrinoLyslyslys ins-Chain;

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1. Identification and Chaeracterizatio nof Novel tPA-and Plasminogen-Binding Sites within Fibrin(ogen) #alpha#C-Domains [J] . Galina Tsurupa, Leonid Medved Biochemistry . 2001,第3期

机译：纤维蛋白（基因）＃alpha＃C域内的新型tPA和纤溶酶原结合位点的鉴定和特征鉴定
2. Investigation of residues in the fibrin(ogen) gamma chain involved in tissue plasminogen activator binding and plasminogen activation. [J] . Wilhelm SE, Lord ST Blood coagulation & fibrinolysis: an international journal in haemostasis and thrombosis . 2004,第6期

机译：研究纤维蛋白（原）γ链中与组织纤溶酶原激活物结合和纤溶酶原激活有关的残基。
3. Interaction of fibrin(ogen) with apolipoprotein(a): Further characterization and identification of a novel lysine-dependent apolipoprotein(a)-binding site within the gamma chain 287-411 region [J] . Tsurupa G, Yakovlev S, Pechik I, Biochemistry . 2006,第35期

机译：纤维蛋白（原）与载脂蛋白（a）的相互作用：γ链287-411区域内新型赖氨酸依赖性载脂蛋白（a）结合位点的进一步表征和鉴定
4. Side-Chain Losses in Electron Capture Dissociation Improves the Identification of Pt(II)-Binding Sites on Peptides and Proteins [C] . Huilin Li, Jonathon R. Snelling, Mark P. Marrow, American Society for Mass Spectrometry Conference on Mass Spectrometry and Allied Topics . 2013

机译：电子捕获解离中的侧链损失改善了肽和蛋白质上的Pt（II）的识别位点
5. A structure-function analysis of the lysine-binding kringle 2 domains of human tissue-type plasminogen activator and human plasminogen by strategic mutagenesis. [D] . Nilsen, Stephanie Lynn. 1999

机译：通过战略诱变对人体组织型纤溶酶原激活物和纤溶酶原的赖氨酸结合kringle 2域的结构功能分析。
6. Interaction between plasminogen activator inhibitor type 1 (PAI-1) bound to fibrin and either tissue-type plasminogen activator (t-PA) or urokinase-type plasminogen activator (u-PA). Binding of t-PA/PAI-1 complexes to fibrin mediated by both the finger and the kringle-2 domain of t-PA. [O] . O F Wagner, C de Vries, C Hohmann, 1989

机译：与纤维蛋白结合的1型纤溶酶原激活物抑制剂（PAI-1）与组织型纤溶酶原激活物（t-PA）或尿激酶型纤溶酶原激活物（u-PA）之间的相互作用。 t-PA / PAI-1复合物与t-PA的手指和kringle-2结构域介导的纤维蛋白结合。
7. Interaction between plasminogen activator inhibitor type 1 (PAI-1) bound to fibrin and either tissue-type plasminogen activator (t-PA) or urokinase-type plasminogen activator (u-PA). Binding of t-PA/PAI-1 complexes to fibrin mediated by both the finger and the kringle-2 domain of t-PA. [O] . Wagner, O F, de Vries, C, Hohmann, C, 1989

机译：与纤维蛋白结合的1型纤溶酶原激活物抑制剂（PAI-1）与组织型纤溶酶原激活物（t-PA）或尿激酶型纤溶酶原激活物（u-PA）之间的相互作用。 t-PA / PAI-1复合物与t-PA的手指和kringle-2结构域介导的纤维蛋白结合。
8. Fibrin(ogen) -Mediated Extracellular Transport of Breast Cancer Cells by Macrophages: A New Idea Regarding Metastasis [R] . Retzinger, G. S. 2004

机译：纤维蛋白（ogen）介导巨噬细胞对乳腺癌细胞的细胞外转运：关于转移的新思路

Identification of the binding site for plasminogen kringle 5 in the α-chain of fibrin(ogen) D-fragment

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