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首页> 外文期刊>Thoracic cancer. >CircAGFG1 sponges miR‐203 to promote EMT and metastasis of non‐small‐cell lung cancer by upregulating ZNF281 expression
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CircAGFG1 sponges miR‐203 to promote EMT and metastasis of non‐small‐cell lung cancer by upregulating ZNF281 expression

机译：CircagFG1海绵MIR-203通过上调ZNF281表达来促进非小细胞肺癌的EMT和转移

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The circRNA circAGFG1 is reported to be important in triple-negative breast cancer progression. However, the mechanism of circAGFG1 in non-small-cell lung cancer (NSCLC) remains unknown. In this study, expression of circAGFG1 was determined by real-time PCR in 20 pairs of NSCLC tissues and adjacent tissues. Next, functional experiments with circAGFG1 were performed in vitro to evaluate the role of circAGFG1 in tumor metastasis and growth. Meanwhile, a dual luciferase reporter assay, RNA pull-down and RNA immunoprecipitation experiments were used to explore the interaction between circAGFG1 and miR-203. Our results revealed that expression levels of circAGFG1 and miR-203 are upregulated in non-small-cell lung cancer tissues. CircAGFG1 enhances NSCLC cell proliferation, invasion, migration and epithelial-mesenchymal transition in vitro. Mechanistic analyses indicated that circAGFG1 acts as a sponge for miR-203 to repress the effect of miR-203 on its target, ZNF281. In conclusion, our study suggests that circAGFG1 promotes NSCLC growth and metastasis though a circAGFG1/miR-203/ZNF281 axis and may represent a novel therapeutic target. ? 2019 The Authors. Thoracic Cancer published by China Lung Oncology Group and John Wiley & Sons Australia, Ltd.
机译：据报道，CircRNA CircAMFG1在三阴性乳腺癌进展中是重要的。然而，在非小细胞肺癌（NSCLC）中的CIRCAMFG1的机制仍然未知。在该研究中，通过20对NMSCLC组织和相邻组织中的实时PCR来确定CircagFG1的表达。接下来，在体外进行用CircAGFG1的功能实验，以评估CircAMG1在肿瘤转移和生长中的作用。同时，使用双荧光素酶报告器测定，RNA下拉和RNA免疫沉淀实验用于探讨CircagFG1和miR-203之间的相互作用。我们的结果表明，CircagFG1和miR-203的表达水平在非小细胞肺癌组织中上调。 CircagFG1增强了体外的NSCLC细胞增殖，侵袭，迁移和上皮间过渡。机械分析表明，CircagFG1充当MIR-203的海绵，以抑制miR-203对其目标ZnF281的影响。总之，我们的研究表明，CircAMFG1促进了NSCLC生长和转移，尽管CircagFG1 / miR-203 / ZNF281轴，并且可以代表一种新的治疗目标。 ？ 2019年的作者。中国肺部肿瘤集团和约翰瓦里和儿子澳大利亚发表的胸癌

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