PLLA Synthesis and Nanofibers Production: Viability by Human Mesenchymal Stem Cell from Adipose Tissue

M.V. Xavier; M.F. Macedo; A.C.B. Benatti; A.L. Jardini; A.A. Rodrigues; M.S. Lopes; C.S. Lambert; R.M. Filho; P. Kharmandayan

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PLLA Synthesis and Nanofibers Production: Viability by Human Mesenchymal Stem Cell from Adipose Tissue

机译：PLLA合成和纳米纤维生产：来自脂肪组织的人间充质干细胞的活力

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The absorbable polyacid is one of the most used and studied materials in tissue engineering. This work synthesized a poly (L-lactic acid) (PLLA) through ring-opening polymerization and produced with it nanofibers by the electrospinning process. The PLLA was analyzed by FTIR and its cytotoxicity was evaluated by the MTT assay and Live/Dead? (Molecular Probes). The tests were performed in contact with human mesenchymal cells at varying times. The high rates of viability and proliferation of cells in contact with the PLLA shown by MTT and Live/Dead? tests demonstrate that this PLLA is a biocompatible material. There was also the successful production of electrospinning nanofibers, which can be converted for specific biomedical applications in the future.

机译：可吸收的多酸是组织工程中最受使用和研究的材料之一。该工作通过开环聚合合成聚（L-乳酸）（PLLA）并通过静电纺丝工艺用其用IT纳米纤维制备。通过FTIR分析PLLA，其细胞毒性由MTT测定和活/死亡评估？（分子探针）。在不同时间以与人间充质细胞接触的测试进行测试。与MTT和Live / Dead所示的PLLA接触的细胞的高可活力和细胞增殖的高率？测试表明，该PLLA是生物相容性的材料。还有耐电纺丝纳米纤维的成功生产，可以在未来转换特定的生物医学应用。

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《Procedia CIRP》 |2016年第5apartaa期|共9页
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M.V. Xavier; M.F. Macedo; A.C.B. Benatti; A.L. Jardini; A.A. Rodrigues; M.S. Lopes; C.S. Lambert; R.M. Filho; P. Kharmandayan;
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1. Effects of Expanded Human Adipose Tissue-Derived Mesenchymal Stem Cells on the Viability of Cryopreserved Fat Grafts in the Nude Mouse [J] . Myung-Soon Ko, Ji-Youl Jung, Il-Seob Shin, International Journal of Medical Sciences . 2011,第3期

机译：扩增的人类脂肪组织间充质干细胞对裸鼠冷冻保存的脂肪移植物活力的影响
2. Tissue source determines the differentiation potentials of mesenchymal stem cells: a comparative study of human mesenchymal stem cells from bone marrow and adipose tissue [J] . Liangliang Xu, Yamei Liu, Yuxin Sun, Stem Cell Research & Therapy . 2017,第1期

机译：组织来源决定了间充质干细胞的分化潜能：来自骨髓和脂肪组织的人间充质干细胞的比较研究
3. Conditioned Media from Human Adipose Tissue-Derived Mesenchymal Stem Cells and Umbilical Cord-Derived Mesenchymal Stem Cells Efficiently Induced the Apoptosis and Differentiation in Human Glioma Cell Lines In Vitro [J] . Chao Yang, Deqiang Lei, Weixiang Ouyang, BioMed research international . 2014,第27期

机译：人类脂肪组织间充质干细胞和脐带来源间充质干细胞的条件培养基有效地诱导人胶质瘤细胞系的凋亡和分化。
4. PLLA Synthesis and Nanofibers production: Viability by Human Mesenchymal Stem Cell from Adipose Tissue [C] . Xavier M. V., Macedo M. F., Benatti A. C. B., CIRP Conference on BioManufacturing . 2016

机译：PLLA合成和纳米纤维生产：来自脂肪组织的人间充质干细胞的活力
5. Analyses of Human Adipose Derived Stem Cells and Human Mesenchymal Stem Cells for Functional Tissue Engineering using Biomimetic Physical Stimuli. [D] . Charoenpanich, Adisri. 2013

机译：使用仿生物理刺激对人体脂肪干细胞和人间充质干细胞进行功能组织工程的分析。
6. Lack of Obvious Influence of PLLA Nanofibers on the Gene Expression of BMP-2 and VEGF during Growth and Differentiation of Human Mesenchymal Stem Cells [O] . Markus D. Schofer, S. Fuchs-Winkelmann, C. Wack, 2009

机译：在人间充质干细胞生长和分化过程中PLLA纳米纤维对BMP-2和VEGF基因表达缺乏明显影响。
7. PLLA Synthesis and Nanofibers Production: Viability by Human Mesenchymal Stem Cell from Adipose Tissue [O] . Xavier M.V., Macedo M.F., Benatti A.C.B., 2016

机译：PLLA合成和纳米纤维生产：来自人体组织的人间充质干细胞的生存能力

PLLA Synthesis and Nanofibers Production: Viability by Human Mesenchymal Stem Cell from Adipose Tissue

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