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首页> 外文期刊>Veterinary and Animal Science >Myringotomy in dogs: Contamination rate from the external ear canal - a pilot study
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Myringotomy in dogs: Contamination rate from the external ear canal - a pilot study

机译：狗的术 - 来自外耳道的污染率 - 试点研究

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Myringotomy for sample collection from the middle ear cavity for cytology and bacterial culture is considered a routine method to diagnose otitis media in dogs. The objective of this study was to determine the rate of contamination of middle ear aspirates with material from the external ear canal obtained by video-otoscopic guided myringotomy. In canine cadavers ( n =?17) free from otitis externa the external ear canals were flushed under video-otoscopic control and a fluorescent dye was instilled. After removal of residual fluid a myringotomy was performed. If air was aspirated, 1?mL of saline was instilled through the same myringotomy needle into the middle ear cavity and re-aspirated. Contamination from the external ear canal was demonstrated by positive fluorescence of the aspirate. Bacterial cultures and cytological examinations of the external ear canals and middle ear cavities were performed. Data from 28 ears under investigation were included. In 19 of 28 middle ear aspirates (67.9%), clear yellow fluorescent fluid was obtained, indicating a contamination from the external ear canal. Microorganisms were detected in 4 of 26 middle ear samples (15.4%) and in 15 of 26 external ear canals (57.7%). Sample collection by myringotomy in this study was associated with a high contamination rate, implying that the suitability of this method for detection of otitis media in patients with concurrent otitis externa is questionable. Furthermore, the potential for iatrogenic spread of pathogenic microorganisms into the middle ear cavity needs to be considered.
机译：用于从中耳腔进行细胞学和细菌培养的样品收集的主要术，被认为是诊断狗中耳炎的常规方法。本研究的目的是确定中耳吸气速率与来自视频 - 耳镜引导的术术的外耳道中的材料。在犬齿尸体（n =α17）中，没有耳炎，外部耳状物在视频 - 耳镜对照下刷新，滴注荧光染料。除去残留的液体后，进行术术。如果吸气空气，将1μlα盐水通过将1μl盐水滴注到中耳腔中并重入耳腔。通过抽吸物的正荧光证明了外部耳道的污染。进行了外耳道和中耳腔的细菌培养和细胞学检查。包括在调查下的28个耳朵的数据。在28个中耳吸气（67.9％）中，获得了透明的黄色荧光流体，表明来自外耳道的污染。在26个中耳样品（15.4％）中的4个中检测到微生物，在26个外耳管中的15个（57.7％）中。本研究中的主要污染率的样品收集与高污染率有关，这意味着这种方法对患有同时性耳炎的患者中耳炎介质的适用性是值得怀疑的。此外，需要考虑致病微生物到中耳腔中的致病微生物的可能性。

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