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首页> 外文期刊>Drug Design, Development and Therapy >Lentinan Inhibits AGE-Induced Inflammation and the Expression of Matrix-Degrading Enzymes in Human Chondrocytes
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Lentinan Inhibits AGE-Induced Inflammation and the Expression of Matrix-Degrading Enzymes in Human Chondrocytes

机译：Lentinan抑制年龄诱导的炎症和人软骨细胞中基质降解酶的表达

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Background: Chondrocyte-mediated inflammation is an important pathological component of osteoarthritis (OA) development. There are currently no therapies that completely reverse the development of OA. Lentinan, a type of polysaccharide derived from Lentinus edodes, has been demonstrated to possess significant anti-viral, anti-cancer, and anti-inflammatory effects, and has been recently used in the treatment of several inflammatory diseases. However, little research has focused on the pharmacological effect of lentinan in human OA. Materials and Methods: We evaluated the anti-inflammatory and anti-ROS effects of lentinan in SW1353 chondrocytes treated with AGEs using real-time polymerase chain reaction (PCR), enzyme-linked immunosorbent assay (ELISA), and the nitro oxide-specific stain DAF-FM DA. The regulatory effects of lentinan on NF-κB and MAPK p38 signaling were investigated via promoter assay and Western blot analysis. Results: We found that lentinan inhibits the production of pro-inflammatory cytokines, including IL-1β, TNF-α, IL-8 and the secretion of PGE2 and NO, by reducing the expression of COX-2 and iNOS in AGE-challenged chondrocytes. Lentinan also reduces AGE-induced increased expression of matrix metalloproteinases-1, ? 3, and ? 13 (MMP-1, MMP-3, MMP-13). Furthermore, lentinan has a similar effect on a disintegrin and metalloproteinase with thrombospondin motifs-4 and ? 5 (ADAMTS-4, ADAMTS-5). Mechanistically, lentinan reduces the activation of NF-κB. Conclusion: Our findings indicate that lentinan shows a protective effect against AGE-induced inflammatory response in chondrocytes. These findings suggest that lentinan is a promising agent for the treatment of OA that could be used as a dietary supplement for patients with OA.
机译：背景：软骨细胞介导的炎症是骨关节炎（OA）发育的重要病理成分。目前没有完全逆转OA的发展的疗法。 Lentinan是一种衍生自Lentinus edodes的多糖，已经证明具有显着的抗病毒，抗癌和抗炎作用，并且最近用于治疗几种炎症疾病。然而，小型研究侧重于Lentinan在人OA中的药理作用。材料和方法：通过使用实时聚合酶链反应（PCR），酶联免疫吸附测定（ELISA）和硝基氧化物特异性染色，评估了在用年龄处理的SW1353软骨细胞中的抗炎和抗ROS疗效。 DAF-FM DA。通过启动子测定和Western印迹分析研究了Lentinan对NF-κB和MAPK P38信号传导的调节作用。结果：我们发现Lentinan抑制了促炎细胞因子的产生，包括IL-1β，TNF-α，IL-8和PGE2的分泌，通过减少年龄攻击的软骨细胞中COX-2和Inos的表达。香菇还减少了年龄诱导的基质金属蛋白酶酶-1的增加表达，？ 3，和？ 13（MMP-1，MMP-3，MMP-13）。此外，Lentinan对具有血压出蛋白基序-4和血栓间素基序和金属蛋白酶的效果类似的效果。 5（Adamts-4，Adamts-5）。机械地，Lentinan减少了NF-κB的活化。结论：我们的研究结果表明，Lentinan表明，对软骨细胞的年龄诱导的炎症反应显示出保护作用。这些发现表明，Lentinan是治疗OA的oA的有希望的剂，可用于OA患者的膳食补充剂。

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