...
  • 主题
高级搜索  >
历史搜索 清空历史
首页> 外文期刊>Journal of genetics >Genomic marker assisted identification of genetic loci and genes associated with variation of grain zinc concentration in rice
【24h】

Genomic marker assisted identification of genetic loci and genes associated with variation of grain zinc concentration in rice

机译：基因组标记物辅助鉴定水稻籽粒锌浓度变异的遗传基因座和基因

获取原文
           
页面导航
  • 摘要
  • 著录项
  • 相似文献
  • 相关主题

摘要

A study was conducted to examine the genetic divergence and to determine the genetic loci and genes associated with natural variation of grain zinc (Zn) concentration among 28 landraces, improved varieties and advanced breeding lines of rice using candidate gene specific primers. Field evaluation of the experimental material was conducted in randomized block design with three replications and Zn content in unpolished grains of the entries was determined by addition of nitric acid and perchloric acid (1:3) following the procedure ofdiacid digestion method. Statistical analysis revealed the exploitable extent of variability with respect to grain Zn concentration among the entries. Eighteen entries were selected from the two extremes of grain Zn distribution range and subjected to molecular profiling using a panel of 14 candidate genes specific 12 reported and 14 designed primer pairs. Only eight (OsZIP1-1, OsZIP3a, OsZIP4a, OsZIP5-3, OsZIP7-2, OsZIP8b, OsNRAMP7 and OsNAAT1) reported and eight (OsZIP3K, OsZIP4K, OsZIP5K, OsZIP7K, OsNRAMP7K, OsNAAT1K, OsNACK and OsYSL14K) designed primers generated polymorphic amplified products showing sequence length variation due to targeted amplification of candidate genes specific genomic regions. Ample genetic differentiation and divergence were revealed among the entries, which were accommodated into similarity coefficient-based six clusters, remarkably consistent with grain Zn concentrationof the entries. Hierarchical classification pattern of entries was almost completely corroborated by principal co-ordinate analysisbased spatial distribution pattern of their genetic profiles. Molecular analysis based on candidate genes specific primers appeared to be an efficient approach for the elucidation of genetic differentiation and divergence in relation to variation of grain Zn concentration among entries. Hence, these markers can be effectively and efficiently utilized for grain Zn concentration related discrimination of rice genotypes and selection of parental genotypes for grain Zn biofortification. Microsatellites were detected within the candidate genes and amplicons, thereby providing a basis to deduce that the repeat sequence length variation in candidate genes may be a role player in the differential grain Zn accumulation in rice varieties. Single marker analysis established the association of OsNACK, OsZIP1-1, OsNRAMP7 and OsNRAMP7K with grain Zn concentration. Thus, these four markers can be effectively used in marker-assisted selection programme for grainZn biofortification in rice.
机译：进行了研究以检查遗传分歧，并确定使用候选基因特异性引物在28个地位，改善品种和晚期水稻的籽粒锌（Zn）浓度的自然变化相关的遗传基因群和基因。在随机嵌段设计中对实验材料进行实验材料的田间评估，并通过在DiCID消化方法的方法后加入硝酸和高氯酸（1：3）测定条目的三种重复和Zn含量。统计分析显示了条目中谷物Zn浓度的可变异性的可变异程度。从谷粒Zn分布范围的两个极端选择十八个条目，并使用特异性12个候选基因的面板进行分子分析，报告和14个设计的底漆对。仅报告八（oszip1-1，oszip3a，oszip4a，oszip5-3，oszip7-2，oszip8b，osnramp7和osnaat1）和八个（oszip3k，oszip4k，oszip5k，oszip7k，osnramp7k，osnaat1k，osnack和osysl14k）设计了引物产生的多晶型放大显示序列长度变化的产品由于候选基因特异性基因组区域的靶向扩增。在条目中揭示了充分的遗传分化和分歧，其被容纳在基于相似系数的六个簇中，与条目的晶粒Zn浓度相一致。各个条件分析分析的遗传谱的空间分布模式几乎完全证实条目的分层分类模式。基于候选基因的分子分析特异性引物似乎是阐明遗传分化和与条目中谷物Zn浓度的变异的致异质的有效方法。因此，可以有效地和有效地利用这些标志物用于水稻基因型的晶粒锌浓度相关判断以及谷物Zn生物凝固的父母基因型的选择。在候选基因和扩增子内检测到微卫星，从而向推导求助于候选基因的重复序列长度变化可以是水稻品种中差分谷物Zn积累的角色扮演者。单个标记分析建立了粒子Zn浓度的Osnack，Oszip1-1，Osnramp7和Osnramp7k的关联。因此，这四个标记可以有效地用于水稻中的麦片生物融合的标记辅助选择程序中。

著录项

相似文献

  • 外文文献
  • 中文文献
  • 专利
获取原文

客服邮箱：kefu@zhangqiaokeyan.com

京公网安备：11010802029741号 ICP备案号：京ICP备15016152号-6 六维联合信息科技 (北京) 有限公司©版权所有

  • 客服微信

  • 服务号