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首页> 外文期刊>Journal of genetics >Assessment of genetic diversity and relationships among Salvia species using gene targeted CAAT box-derived polymorphism markers
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Assessment of genetic diversity and relationships among Salvia species using gene targeted CAAT box-derived polymorphism markers

机译：使用基因靶向CAAT盒衍生的多态性标记评估萨尔维亚物种遗传多样性和关系的评估

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To detect the genetic variation and relationships among different Salvia ecotypes/species, the gene targeted CAAT boxderived polymorphism (CBDP) markers were employed in terms of their efficiency. In this study, 25 CBDP primers amplified a total of 323 different polymorphic fragments that discriminate all 26 Salvia ecotypes/species and produced an informative and differentiated dendrogram and population structure. The CBDP markers were found to be effective in Salvia genetic diversity estimation with regard to the averages polymorphism (100%), polymorphism information content (PIC = 0.89), marker index (MI = 4.5) and the effective multiplex ratio (EMR = 5.01) which were higher than other reported markers on Salvia. The extent of heterozygosity (0.034a?¤Ha?¤0.223) and Shannon index (0.042a?¤Ia?¤0.278) indicated a high level of genetic variation among Salvia species. The species containing the highest basic chromosome number (X = 12) revealed the highest values for the number of different (Na) and effective (N e) alleles, Shannon index (I), and heterozygosity (H). Additionally, the tetraploid species showed high values of N a, Ne, I and H compared to the diploid species. Mean of gene differentiation (Gst) among Salvia species was 0.792, and the estimation of gene flow (Nm) was 0.13, indicating high genetic differentiation. Remarkably, similar results were obtained from the principal co-ordinate analysis (PCoA) as compared with the cluster analysis, in which all different Salvia species formed individual groups. In conclusion, because the CBDP markers are derived from the gene containing regions of the genome, consequently, the high genetic diversity among studied Salvia species would be more useful for crop improvement programmes, such as hybridization between species and QTL mapping. The potential of CBDPs for analysing the phylogeny and genetic diversity of Salvia species is another key result with practical implications.
机译：为了检测不同丹参/物种之间的遗传变异和关系，在其效率方面采用靶向Caat诱导的多态性（CBDP）标志物。在该研究中，25个CBDP引物扩增了总共323种不同的多态性片段，可区分所有26个丹参/物种，并产生了一种信息和分化的树枝状和群体结构。发现CBDP标记有效地在萨尔维亚遗传多态性（100％），多态性信息含量（PIC = 0.89），标记指数（MI = 4.5）和有效多路复用比（EMR = 5.01）中有效这比丹参的其他报告标志物高。杂合子的程度（0.034A？¤Ha？¤0.223）和香农指数（0.042A？¤？?0.278）表明了丹参物种的高水平遗传变异。含有最高染色体数量（X = 12）的物种揭示了不同（NA）和有效（N e）等位基因，香农指数（I）和杂合子（H）的最高值。另外，与二倍体物种相比，四倍体物种显示出高值N，Ne，I和H. Salvia物种中基因分化（GST）的平均值为0.792，基因流动（NM）的估计为0.13，表明高遗传分化。值得注意的是，与聚类分析相比，从主坐标分析（PCOA）获得了类似的结果，其中所有不同的丹参物种形成单个组。总之，因为CBDP标记衍生自含基因组的含基因区域，因此研究的Salvia物种之间的高遗传多样性对于作物改善计划更有用，例如物种与QTL映射之间的杂交。 CBDPS用于分析Salvia物种的系统发育和遗传多样性的潜力是具有实际影响的另一个关键结果。

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