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首页> 外文期刊>Journal of genetics >Molecular characterization and expression pattern analysis of a novel stress-responsive gene a??BeSNAC1a?? in Bambusa emeiensis
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Molecular characterization and expression pattern analysis of a novel stress-responsive gene a??BeSNAC1a?? in Bambusa emeiensis

机译：一种新的应激响应基因A的分子表征和表达模式分析BESNAC1A ??在Bambusa Emeiensis.

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NAC transcription factors (TFs) are master regulators of environmental stresses exerting a crucial role in plant growth and development. However, the studies on NAC TFs from Bambusa emeiensis are scarce. In this investigation, a novel gene from B. emeiensis encoding NAC protein was cloned and characterized. The gene was isolated based on the amino acid sequence data of stress-responsive SNAC1 of rice, named a??BeSNAC1 (accession no. MG763922)a??. The full-length sequence of 1681 bp was found to contain an open-reading frame of 912 bp that encode a protein of 303 amino-acid residues. The multiple protein sequence alignments unveiled that BeSNAC1 contains a typical NAC domain. Additionally, the phylogenetic analysis showed that the corresponding protein belonged to the SNAC group, as it cladded with SNAC1, HvSNAC1, TaNAC2, SbSNAC1 and ZmSNAC1 proteins. Transactivation and subcellular localization assay disclosed that BeSNAC1 is a transcriptional activator localized in the cell nucleus.Moreover, the time-dependent expression pattern of BeSNAC1 was profiled under abscisic acid (ABA), polyethylene glycol 6000 (PEG-6000), NaCl, H2O2 and Na2SO4 treatments via a quantitative real-time polymerase chain reaction. The results revealed that the expression of BeSNAC1 was significantly upregulated in all treatments, a significant difference was observed under H2O2, NaCland ABA (P 0.001) and PEG and Na2SO4 (P 0.01) treatments, respectively. Conclusively, our findings provide evidence that a??BeSNAC1a?? is a nuclear protein that might act as part of the transcription regulation complex and is involved in the ABA signalling pathway and abiotic stress tolerance mechanisms in B. emeiensis.
机译：NAC转录因子（TFS）是环境压力的主监管机构对植物生长和发育中的至关重要作用。然而，来自Bambusa Emeiensis的NAC TFS的研究是稀缺的。在该研究中，克隆并表征了来自B. eMeiensis的新基因。基于稻米的应激响应SnaC1的氨基酸序列数据分离出该基因，命名为a ?? besnac1（登录号Mg763922）a ??。发现1681bp的全长序列含有912bp的开放式读数框，其编码303个氨基酸残基的蛋白质。揭开的多种蛋白质序列比对含有典型的NAC结构域。另外，系统发育分析表明，相应的蛋白质属于SNAC组，因为它包覆有SNAC1，HVSNAC1，TANAC2，SBSNAC1和ZMSNAC1蛋白。转移和亚细胞定位检测公开了，BESNAC1是局部在细胞核中局部化的转录活化剂。罗纳酸的时间依赖性表达模式在隔离酸（ABA），聚乙二醇6000（PEG-6000），NaCl，H 2 O 2和NaCl通过定量实时聚合酶链反应的Na 2 SO 4处理。结果表明，在所有治疗中显着上调BESNAC1的表达，分别在H 2 O 2，尼卡兰（P <0.001）和PEG和NA2SO4（P <0.01）处理下观察到显着差异。结论，我们的调查结果提供了一个证据表明a ?? besnac1a ??是一种核蛋白质，可作为转录调节综合体的一部分，并参与B. emeiensis中的ABA信号通路和非生物胁迫耐受机制。

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