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首页> 外文期刊>Journal of genetics >Transcriptome profiling of four candidate milk genes in milk and tissue samples of temperate and tropical cattle
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Transcriptome profiling of four candidate milk genes in milk and tissue samples of temperate and tropical cattle

机译：四种候选牛奶基因的转录组分析在温带和热带牛的牛奶和组织样本中的四种候选乳基因

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The expression of four genes involved in milk regulation and production in bovine milk and tissue samples profiled using quantitative PCR to identify differential gene expression. Our goal focussed on the differential mRNA expression of milk genes (KCN, PRL, BLG and PIT-1) in milk samples and different tissues from four different breeds of ecologically adapted and geographically separated cattle species. The mRNA expression identified the four milk genes understudiedmost upregulated in mammary gland and milk samples as compared with other tissues. The expression of PIT-1 gene in the brain identified to have influenced the expression of PRL and K-CN in the mammary and milk samples. Among the four genes, PRL had the highest mRNA expression (144.19-fold change) in Holstein followed by K-CN with 100.89-fold change, while the smallest relative expression for most genes in this study are in the range from 0.79 to 7.35-fold difference. White Fulani cattle was identified to have a higher expression for K-CN, PRL and BLG compared with Angus and Ndama cattle, while Holstein cattle is on top of the list on the basis of the gene expression and gene regulation for all the four genes in this study. Also, White Fulani and Holstein are in the same cluster based on their mRNA expression for milk genes. Our data showed the first evidence of the molecular identification of indigenous White Fulani cattle ofhaving potential for higher milk production.
机译：牛奶中涉及牛奶调节和生产中的四种基因的表达和使用定量PCR分析的组织样品以鉴定差异基因表达。我们的目标侧重于牛奶样品（KCN，PRL，BLG和PIT-1）的差异mRNA表达，以及来自四种不同品种的生态适应和地理上分离的牛种类的不同组织。与其他组织相比，mRNA表达鉴定了在乳腺和乳汁中最高升高的四种乳基因。诱导脑内的坑1基因的表达影响了PRL和K-CN在乳腺和牛奶样品中的表达。在四个基因中，PRL在Holstein中具有最高的mRNA表达（144.19倍变化），然后具有100.89倍的变化，而本研究中大多数基因的最小相对表达在0.79至7.35的范围内。折叠差异。与Angus和Ndama牛相比，鉴定了白色富兰尼牛，对K-CN，PRL和BLG具有更高的表达，而荷斯坦牛在列表的基础上基于基因表达和所有四种基因的基因调节这项研究。此外，白富福氏素和孔斯坦在同一簇中基于它们的mRNA表达用于乳基因。我们的数据显示了第一个证据表明土着白色富拉牛的分子鉴定，牛奶产量较高的潜力。

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