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Molecular profiling of blast resistance genes and evaluation of leaf and neck blast disease reaction in rice

机译:水稻抗抗原基因的分子分析及叶片颈部爆炸疾病反应评价

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In the present investigation, phenotypic evaluation of blast disease reaction was conducted at Ponnampet and Mandya districts of Karnataka, India, which indicated that the rice varieties such as IR64, Jaya, KMP153, IR30864, Mandya Sona-1, Mandya Sona-2, KCP-1, Dodda Byra, and Malgudi Sanna were susceptible to both leaf and neck blasts. Further, the rice varieties that were resistant to leaf blast such as KMP200, DHMAS70Q164-1b, Karibatta, Coimbatore Sanna and others showed susceptible reaction to neck blast only. In contrast, the varieties such as JyothixBR2655, Punkutt Kodi, Sirsi, 222 and Gangadale which were resistant to neck blast were found to be susceptible to leaf blast also. Only one variety, BR2655 showed resistance to both leaf and neck blast diseases. The genotypic studies using simple sequence repeat markers showed that the analysis of the distribution of resistance genes and genotyping of the selected rice varieties, and traditional rice varieties from different ecological regions with allele specific markers helped to identify 20 major blast resistance genes. The individual gene frequencies of the 20 major rice blast resistance genes varied from 10.34 to 100%. Less and more frequency of resistance gene distribution occurred in Pi9 and Pizt gene, respectively. The result of this study would help to create strategies for improving rice blast resistance through genetic studies and planta??pathogen interaction.
机译:在目前的研究中,在印度的Ponnampet和Mandya地区进行了爆炸疾病反应的表型评估,该地区表示IR64,Jaya,KMP153,IR30864,Mandya Sona-1,Mandya Sona-2,KCP等水稻品种-1,Dodda Byra,Malgudi Sanna易患叶子和颈部爆炸。此外,对叶片爆炸的水稻品种如Kmp200,Dhmas70q164-1b,karibatta,coimbatore sanna等易受影响的反应仅对颈部爆炸进行了影响。相比之下,诸如JyothixBr2655,Punkutt Kodi,Sirsi,222和Bangadale的品种被发现也易于造成叶片爆炸。只有一种品种,BR2655显示出对叶片和颈部爆炸疾病的抵抗力。使用简单序列重复标记的基因型研究表明,从等级特异性标记物中分析了所选水稻品种的抗性基因和基因分型的分布,以及来自不同生态区域的传统水稻品种有助于鉴定20个主要的抗抗抗原基因。 20个主要稻瘟病抗性基因的个体基因频率从10.34变化到100%。 PI9和PIZT基因分别发生越来越多的抗性基因分布频率。该研究的结果将有助于通过遗传研究和Planta的病原体相互作用产生改善水稻抗抗抗性的策略。

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