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首页> 外文期刊>Journal of genetics >Identification of genomic regions associated with early plant vigour in lentil (Lens culinaris)
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Identification of genomic regions associated with early plant vigour in lentil (Lens culinaris)

机译：扁豆植物早期植物相关基因组区域（晶状体玉米糖）

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Lentil is one of the most important food legume species, however its genetic and genomic resources remained largely uncharacterized and unexploited. In the past few years, a number of genetic maps have been constructed and marker resources have been developed in lentil. These resources could be exploited for understanding the extent and distribution of genetic variation in genus Lens andalso for developing saturated and consensus genetic maps suitable for quantitative trait loci (QTL) mapping and marker-assisted selection.The present study aims to enrich polymerase chain reaction-based linkage map of F10 recombinant inbred lines (RILs) population of 94 individuals derived from cross WA8649090 9 Precoz and identification of QTLs linked to early plant vigour traits. Of the 268 polymorphic markers (93 simple sequence repeats (SSR), three inter-simple sequence repeats (ISSRs) and 172 random amplified polymorphic DNA (RAPDs)), 265 (90 SSRs, three ISSRs and 172 RAPDs) were mapped on seven linkage groups, varying in length between 25.6 and 210.3 cM, coverage of 809.4 cM with an average marker spacing of 3.05 cM. The study also reported assigning of 24 new cross-genera SSRs of Trifolium pratense on the present linkage map. The RILs along with the parents were screened for shoot length, root length, seedling length, dry weight, number of leaves and number of branches based on two replications under polyhouse conditions. A QTLhotspot consisting of six QTLs for shoot length (cm), root length (cm) and seedling length (cm) was observed between a map distances of56.61 and 86.81 cM on LG1.
机译：扁豆是最重要的食物豆科物种之一，但其遗传和基因组资源仍然大部分是无表特征和未批准的。在过去几年中，已经建立了许多遗传图谱，并且在扁豆上已经开发了标记资源。可以利用这些资源来理解，用于了解适用于定量性状基因座（QTL）测绘和标记辅助选择的饱和和共识遗传图中遗传变异的范围和分布。目前的研究旨在丰富聚合酶链式反应 - 基于F10重组近交系（RILS）的连杆地图94个源自交叉WA8649090 9的群体94个体，并鉴定与早期植物活力性状相关的QTLS。在268个多晶型标记物（93个简单的序列重复（SSR）中，三种简单的序列重复（ISSRS）和172个随机扩增的多态性DNA（RAPDS）），265（90SSR，三个ISSRS和172个RONDS）被映射到七连杆上组，长度在25.6和210.3厘米之间，覆盖率为809.4厘米，平均标记间距为3.05厘米。该研究还报告了在目前的联系地图上分配了24个新的Trifolium PraTense。基于多屋条件下的两种复制，筛选群体与父母一起进行射击长度，根长度，幼苗长度，干重，叶片数和分支数。在LG1上的56.61和86.81cm的地图距离之间观察到由射击长度（cm），根长度（cm）和幼苗长度（cm）组成的qtlhothotpot。

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