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首页> 外文期刊>Journal of King Saud University >Analysis of aflatoxins in foods retailed in Saudi Arabia using immunoaffinity column cleanup and high-performance liquid chromatography-fluorescence detection
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Analysis of aflatoxins in foods retailed in Saudi Arabia using immunoaffinity column cleanup and high-performance liquid chromatography-fluorescence detection

机译：利用免疫亲安柱清理和高效液相色谱 - 荧光检测分析沙特阿拉伯零售食品中的黄曲霉毒素

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A liquid–liquid extraction based high performance liquid chromatography-fluorescence method has been proposed for the quantitation of naturally producing aflatoxins in nuts and nut based products. Aflatoxins extraction from nuts and nut products was involved methanol – water extraction, immunoaffinity column cleanup followed by post column derivatization technique. The gel contents of monoclonal antibody of the column are very specific to the toxins of interest, which makes the extraction experiment rapid, simple, highly specific and sensitive. Additionally, the improve concentration of toxins from complex nuts and nut product matrices helps to reduced chromatography interference and lower limit of detection. The proposed method was authenticated to evaluate the performance in terms of linearity of the calibration, limit of detection, recovery, run-to-run (repeatability) and day-to-day precision (reproducibility). Good quality parameters with LOD between 0.003 and 0.007?ppb, recovery from 89 to 98%, linearity in the range of 0.02 to 100?ppb (r2?>?0.989) and precisions with RSDs were achieved below 4.5% at concentration level of 1.0 and 30.0?ppb. The developed method was effectively used to the analysis of aflatoxins (AFB1,AFB2,AFG1,and AFG2) in twenty nuts and nut products and were found up to 120.1?ng/mL AFB1and 11.2?ppb AFB2in few of the samples.
机译：已经提出了一种液 - 液的高效液相色谱 - 荧光法，用于定量天然生产的螺母和基于螺母的产品中的黄曲霉毒素。从螺母和螺母产品中提取的黄曲霉毒素涉及甲醇 - 水萃取，免疫亲和力柱清理，然后是柱衍生化技术。柱的单克隆抗体的凝胶含量非常特异于感兴趣的毒素，这使得提取实验快速，简单，高度特异性和敏感性。另外，复杂螺母和螺母产物基质的提高毒素的浓度有助于降低色谱干扰和检测下限。验证的方法是通过校准，检测限制，恢复，运行（重复性）和日常精度（再现性）的线性度来评估性能。良好的质量参数，腰部0.003和0.007？PPB，回收率从89升至98％，线性度在0.02至100℃的范围内（R2？>？0.989）和RSD的精度在1.0的浓度水平下达到4.5％以下。和30.0？ppb。在20颗螺母和螺母产品中有效地分析了二十坚果和螺母产品中的黄曲霉毒素（AFB1，AFB2，AFG1和AFG2），并发现最多120.1μg/ mL AFB1和11.2·-PB AFB2。

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