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首页> 外文期刊>Journal of medical bacteriology. >Prevalence of Biofilm Associated Genes in Different Isolates of Staphylococcus aureus
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Prevalence of Biofilm Associated Genes in Different Isolates of Staphylococcus aureus

机译：葡萄球菌不同分离株生物膜相关基因的患病率

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Background: Staphylococcus aureus is the most important etiological agent of bio?lm associated-infections. It is one of the Gram-positive pathogens causing a wide range of nosocomial infections. Genes involved in biofilm formation is a defensive mechanism of this pathogen to combat the host immune response and remain stable in hostile environment. The aim of this study was to investigate prevalence of biofilm associated genes (BAGs). Methods: Eighty samples of Staphylococcus aureus isolates from human infections were collected. Thirteen BAGs including rbf, sigB, sasG, icaA, sarA, icaR, icaD, clfA, clfB, fib, fnbpB, bap and fnbpA were amplified by PCR assay. Results: The prevalence of genes were as follows: sigB (93.7%) and sarA (90%) were the most prevalent BAGs followed by rbf (83.7%), fib (80%), sasG (78.7%), icaR (78.7%), clfB (78.7%), clfA (78.7%), fnbpA (73.7%), icaD (66.2%), icaA (50%), fnbpB (22.5%). However, bap was not detected in any isolate. Conclusion: This study showed that the sensitivity and specificity of PCR is high in the identification of biofilm associated genes in S. aureus. However, researchers need new molecular methods to improve understanding of the exact role of the genes involved in biofilm formation.
机译：背景：金黄色葡萄球菌是生物蛋白最重要的生物染发剂感染的感染。它是革兰氏阳性病原体之一，导致各种神经感染。参与生物膜形成的基因是该病原体的防御机制，用于打击宿主免疫应答并在敌对环境中保持稳定。本研究的目的是调查生物膜相关基因（袋）的患病率。方法：收集来自人感染的葡萄球菌的八十份金黄色葡萄球菌的样品。通过PCR测定扩增，包括RBF，SIGB，SASG，ICAA，SARA，ICAR，ICAD，CLFA，CLFB，FIB，FNBPB，BAP和FNBPA等十三袋。结果：基因的患病率如下：SIGB（93.7％）和SARA（90％）是最普遍的袋子，其次是RBF（83.7％），FIB（80％），SASG（78.7％），ICAR（78.7％） ），CLFB（78.7％），CLFA（78.7％），FNBPA（73.7％），ICAD（66.2％），ICAA（50％），FNBPB（22.5％）。然而，在任何分离物中未检测到弯曲。结论：本研究表明，PCR的敏感性和特异性在鉴定的鉴定金黄色葡萄球菌中生物膜相关基因。然而，研究人员需要新的分子方法来改善对生物膜形成中所涉及的基因的确切作用的理解。

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