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首页> 外文期刊>Journal of Microbiology, Biotechnology and Food Sciences >EVALUATION OF THE SENSITIVITY OF NITROCELLULOSE MEMBRANE LOW-COST ALTERNATIVES USED IN TISSUE BLOT IMMUNOASSAY (TBIA) FOR DETECTION OF SOME PLANT VIRUSES
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EVALUATION OF THE SENSITIVITY OF NITROCELLULOSE MEMBRANE LOW-COST ALTERNATIVES USED IN TISSUE BLOT IMMUNOASSAY (TBIA) FOR DETECTION OF SOME PLANT VIRUSES

机译：评价组织污染免疫测定（TBIA）用于检测一些植物病毒的硝酸纤维素膜低成本替代品的敏感性

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In an attempt of reducing the cost of tissue blot immunoassay (TBIA), some kinds of papers as alternative solid phases in place of nitrocellulose membrane (NCM), were tested for detection of three viruses: Cowpea mosaic virus (CPMV), Tomato mosaic virus (ToMV) and Zucchini yellow mosaic virus (ZYMV). Most of the examined papers developed positive reaction with all tested viruses. Canson paper (150g/m2 and 300g/m2) were sensitive for detection of the tested viruses as NCM and persist in good condition during the processing. Filter paper (Whatman No. 1), local drawing paper (60g/m2), photocopy paper (80g/m2), (56g/m2) and (70g/m2) were very weak and need to taking care in handling. High glossy photo paper (200g/m2) was not effective however, it did not show any infected or healthy tissue print. Good results were obtained when both faces of NCM and Canson paper (300g/m-2) were printed with all tested viruses. TBIA could detect CPMV in infected leaves using the unused face of NCM previously processed with 7 or 11 years ago. CPMV, ToMV and ZYMV were detected 2, 4, 6, 8 and16 days after inoculation with NCM and Canson paper (300g/m-2). Also, the tested viruses were detected in leaf, petiole, stem and root of infected plants. ToMV could be detected using NCM and Canson paper (300g/m-2) in naturally infected tomato plant samples.
机译：试图降低组织污染物免疫测定（TBIA）的成本，测试某些类型的纸张作为替代固体相取代硝酸纤维素膜（NCM），用于检测三种病毒：豇豆镶嵌病毒（CPMV），番茄马赛克病毒（TOMV）和西葫芦黄色马赛克病毒（ZYMV）。大多数检查纸与所有测试病毒产生阳性反应。 Canson纸（150g / m 2和300g / m 2）对测试病毒的敏感性敏感，在加工过程中持续存在良好状态。滤纸（Whatman No.1），局部绘图纸（60g / m 2），复印纸（80g / m 2），（56g / m 2）和（70g / m 2）非常弱，需要进行处理。高光泽的照片纸（200g / m2）无效，它没有显示任何感染或健康的组织印刷。当NCM和CANSON纸（300g / m-2）的两张面部用所有测试病毒印刷时，获得了良好的结果。使用先前在7或11年前处理的NCM的未使用面，TBIA可以检测感染叶片中的CPMV。用NCM和CANSON纸接种后检测到2,4,6,8和16天的CPMV，TOMV和ZYMV（300g / m-2）。此外，在感染植物的叶片，叶柄，茎和根中检测到测试病毒。可以使用NCM和Canson纸（300g / m-2）在天然感染的番茄植物样品中检测到TIMV。

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