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Biofilm sensitivity of seven Streptococcus mutans strains to different fluoride levels

机译:生物膜血液敏感性七种链球菌变异菌株对不同的氟化物水平

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The effect of fluoride concentrations in dental products could be different depending on the Streptococcus mutans strain. The aim of this study was to investigate the effect of different fluoride concentrations corresponding to dental products on biofilm formation and metabolic activity of S. mutans strains. Seven S. mutans strains (UA159, A32-2, NG8, 10449, UA130, LM7, and OMZ175) were inoculated into 96-well microtiter plates and were tested with various concentrations of sodium fluoride (0.0, 1.0, 1.56, 3.13, 6.25, 12.5, 25, 50, 100, 125, 175, 225, 275, 625, 1,250, 2,250, and 5,500 ppm) for inhibition of biofilm formation and bacterial metabolic activity by recording absorbance values followed by scanning electron microscope (SEM) images. Data were analyzed by one-way analysis of variance and Tukey’s tests (α = 5%). Significantly more (p≤0.05) biofilm mass in the presence of fluoride was produced by A32-2 and NG8. UA130, LM7, and OMZ175 were more sensitive to increased fluoride and demonstrated...
机译:牙品浓度在牙科产品中的效果可能取决于链球菌变形菌株的不同。本研究的目的是探讨不同氟化物浓度对应于牙科产品对生物膜形成和S. mutans菌株的代谢活性的影响。将七种调节菌株(UA159,A32-2,NG8,10449,UA130,LM7和OMZ175)接种到96孔微量滴定板中,并用各种浓度的氟化钠(0.0,1.0,1.56,3.13,6.25 ,12.5,25,50,100,125,175,225,275,625,1250,2,250和5,500ppm)通过记录吸光度值,然后通过扫描电子显微镜(SEM)图像来抑制生物膜形成和细菌代谢活性。通过单向分析和Tukey的测试(α= 5%)进行分析数据。在氟化物存在下显着更多(p≤0.05)生物膜质量通过A32-2和NG8制备。 UA130,LM7和OMZ175对氟化物增加更敏感,并证明......

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