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首页> 外文期刊>Journal of Pharmacy and Bioallied Sciences >Assessment of Quality of Platelet-Rich Plasma Produced With Desktop Centrifuge and Comparison With Standardized Commercially Available Platelet-rich plasma
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Assessment of Quality of Platelet-Rich Plasma Produced With Desktop Centrifuge and Comparison With Standardized Commercially Available Platelet-rich plasma

机译:评估桌面离心机生产的富含血小板富含等离子体的质量,并与标准化市售的富含血小板等离子体进行比较

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The aim of this study was to assess the quality of platelet-rich plasma (PRP) produced by in-house desktop centrifuge method and compare it with that of standardized commercial PRP.Materials and Methods:REMI desktop centrifuge was used to prepare PRP and to compare with standardized commercial PRP by calculating the quantity of platelets using Beckman Coulter cell counter in 10 PRP samples and assessing the morphological quality of platelets using JEOL JEM transmission electron microscope (TEM).Statistical Analysis Used:The t test for platelet count in desktop PRP with the test value of therapeutic PRP was 12.618. The P value was <0.001, which was significant statistically. The data followed a normal distribution in normal Q-Q plot for platelet count in desktop centrifuge. So the test samples were not much deviated.Results:The platelet count was lesser than that of standardized commercial PRP. When viewed under JEOL JEM transmission electron microscope, the α granules in platelets were intact and the morphological quality of the PRP was good.Conclusions:With this study, we have determined that the morphological quality of PRP produced by the in-house desktop centrifuge method is comparable to that of standardized commercial PRP. Though the quantity of platelets was less than 1 million cells/μL, the clinical results were good with desired bone formation, thereby providing good avenue for further research.
机译:本研究的目的是评估由内部台式离心机方法生产的富含血小板的血浆(PRP)的质量,并将其与标准化的商业PRP.Materials和方法进行比较:Remi台式离心机用于准备PRP和通过在10个PRP样品中使用Beckman Coulter Cell计数器计算血小板数量并使用JEOL JEM透射电子显微镜(TEM)评估血小板的形态学质量来比较。使用统计分析:桌面PRP中的血小板计数T检验随着治疗性PRP的测试值为12.618。 p值<0.001,其统计学意义。数据遵循桌面离心机的普通Q-Q图中的正常分布。因此,测试样本并不太大偏差。结果:血小板计数比标准化商业PRP的计数小。当在JEOL JEM透射电子显微镜下观察时,血小板中的α颗粒完好无损,PRP的形态学质量很​​好。结论:通过本研究,我们确定了由内部桌面离心机生产的PRP的形态质质量与标准化的商业PRP相当。虽然血小板的数量小于1百万个细胞/μl,但临床结果良好的骨形成良好,从而提供了进一步研究的良好途径。

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