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Occurrence of mycoflora, their association and production of aflatoxin B1 in groundnuts

机译:在地下毒素中的霉菌素,它们的结合和生产的发生

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Groundnut (Arachis hypogaea L.) is an important food crop in Africa which is a source of nutrients and income in rural areas of Zimbabwe. It is considered to be a crop highly susceptible to aflatoxin contamination. Accordingly, the objectives of this study were to understand the presence of mycoflora, their association and the level of contamination by aflatoxins of groundnut from various markets in Zimbabwe. Thirty groundnut samples were purchased randomly from Bulawayo (Shashe and Main market), Gweru (Kudzanayi and Kombayi markets) and Harare (Mbare and Highfield markets). Identi?cation of various fungi was determined using the cultural method on Czapek Dox Agar. Fungi belonging to genera Aspergillus, Mucor, Penicillium and Rhizopus were isolated and characterised from six groundnut markets. Rhizopus species was the most dominant and negatively associated with other fungi species which is attributed to differences in environmental requirements or competition. Aspergillus flavus, Aspergillus fumigatus, Aspergillus niger and Aspergillus parasiticus were identified in groundnut samples with A. flavus being the dominant and found in all markets. The range of AFB1 in groundnut samples analysed using a semi-quantitative immunochromatographic technique was within the safe limits for human consumption according to existing Zimbabwe (5 ppb) regulation. The presence of aflatoxigenic fungi (A. flavus and A. parasiticus) in groundnuts, however, means there is potential for aflatoxin production and fungal proliferation when conditions are favourable.
机译:Groudnut(Arachis Hypogaea L.)是非洲的重要粮食作物,是津巴布韦农村地区的营养和收入来源。它被认为是一种高度易受黄曲霉毒素污染的作物。因此,本研究的目的是了解津巴布韦的各种市场的菌根环洛拉,其关联和污染水平的污染水平。三十个地生样本是从Bulawayo(Shashe和Main Market),Gweru(Kudzanayi和Kombayi Markets)和Harare(Mbare和Highfield Markets)中购买的三十个样品。 identi?使用Czapek Dox琼脂上的文化方法确定各种真菌的阳离子。属于属曲霉属,粘膜,青霉菌和根瘤菌的真菌分离,并用六个地生市场表征。 Rhizopus物种是与其他真菌物种最占优势,归因于环境要求或竞争的差异。曲霉属毛细血管,曲霉属植物,曲霉尼日尔和曲霉属植物,在地生样品中鉴定出与Flavus是主要的,在所有市场中发现。根据现有的津巴布韦(5ppb)调节,在使用半定量免疫层析技术分析的地下室样品中的基因样品的范围内是人们消费的安全限制。然而,在地下素中的黄萎毒性真菌(A.FlaVus和A.AxaSiticus)的存在意味着当条件有利时,有可能对黄曲霉毒素产生和真菌增殖的潜力。

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