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首页> 外文期刊>Journal of Translational Medicine >CMV pp65 and IE-1 T cell epitopes recognized by healthy subjects
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CMV pp65 and IE-1 T cell epitopes recognized by healthy subjects

机译：CMV PP65和IE-1 T细胞表位被健康受试者认识到

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Background Adoptive immune and vaccine therapies have been used to prevent cytomegalovirus (CMV) disease in recipients of hematopoietic progenitor cell transplants, but the nature of T cell responses to CMV have not been completely characterized. Methods Peptide pools and individual peptides derived from the immune-dominant CMV proteins pp65 and IE-1 and antigen-specific, cytokine flow cytometry were used to characterize the prevalence and frequency of CD4+ and CD8+ memory T cells in 20 healthy CMV-seropositive subjects. Results CD8+ T cell responses to pp65 were detected in 35% of subjects and to IE-1 in 40% of subjects. CD4+ T cell responses to pp65 were detected in 50% of subjects, but none were detected to IE-1. Several new IE-1 HLA class I epitopes were identified, including 4 restricted to HLA-C antigens. One region of IE-1 spanning amino acids 300 to 327 was rich in class I epitopes. The HLA class I restrictions of IE-1 peptides were more promiscuous than those of pp65 peptides. Conclusion Since naturally occurring CD4+ and CD8+ T cell responses to pp65 were detectable in many subjects, but only CD8+ T cell responses to IE-1 were detected, pp65 may be better than IE-1 for use in vaccine and adoptive immune therapies.
机译：背景技术采用免疫和疫苗疗法已被用于预防造血祖细胞移植受者中的巨细胞病毒（CMV）疾病，但T细胞对CMV的性质尚未完全表征。方法采用肽池和衍生自免疫显性CMV蛋白PP65和IE-1和抗原特异性的细胞因子流式细胞术的肽池和单个肽在20个健康的CMV血清阳性受试者中表征CD4 +和CD8 +内存T细胞的患病率和频率。结果在35％的受试者和40％受试者中检测到PP65的CD8 + T细胞应答。在50％的受试者中检测到PP65的CD4 + T细胞应答，但没有检测到IE-1。鉴定了几种新的IE-1 HLA类I表位，包括4限制为HLA-C抗原。 IE-1跨越氨基酸300至327的一个区域富含I类表位。 HLA类I限制IE-1肽比PP65肽更混杂。结论由于在许多受试者中检测到对PP65天然存在的CD4 +和CD8 + T细胞反应，但只检测到对IE-1的CD8 + T细胞反应，PP65可以优于IE-1，用于疫苗和采用过的免疫疗法。

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