...
  • 主题
高级搜索  >
历史搜索 清空历史
首页> 外文期刊>BMC Genomics >Isolation of blue-green eggshell pigmentation-related genes from Putian duck through RNA-seq
【24h】

Isolation of blue-green eggshell pigmentation-related genes from Putian duck through RNA-seq

机译：从莆田鸭通过RNA-SEQ分离蓝绿蛋壳色素沉着基因的分离

获取原文
   

获取外文期刊封面封底 >>

       
页面导航
  • 摘要
  • 著录项
  • 相似文献
  • 相关主题

摘要

The diversity of avian eggshell colour plays important biological roles in ensuring successful reproduction. Eggshell colour is also an important trait in poultry, but the mechanisms underlying it are poorly understood in ducks. This study aimed to provide insights into the mechanism of blue-green eggshell colour generation. Here, white-shelled ducks (HBR) and blue-green-shelled ducks (HQR) were selected from Putian black ducks, and white-shelled ducks (BBR) were selected from Putian white ducks. Transcriptional changes in the shell gland were analysed using RNA-sequencing on the Illumina HiSeq 2500. Twenty-seven individual cDNA libraries were sequenced and generated an average of 7.35 million reads per library; 70.6% were mapped to the duck reference genome, yielding an average of 13,794 genes detected, which accounted for approximately 86.39% of all 15,967 annotated duck genes. A total of 899 differentially expressed genes (DEGs) were detected between the HQR and BBR groups, and 373 DEGs were detected between the HQR and HBR groups. We analysed the DEGs in the HQR-vs-BBR and HQR-vs-HBR comparisons. None of these DEGs were directly involved in the eggshell pigmentation process in HQR-vs-HBR, while UDP-glucuronosyltransferase 2A2 (UGT2A2) and UDP-glucuronosyltransferase 1-1-like (UGT1-1-like), which participate in biliverdin breakdown, were two of the DEGs in HQR-vs-BBR. In the RT-qPCR results, delta-aminolevulinic acid synthase 1 (ALAS1) and EPRS glutamyl-prolyl-tRNA synthetase were significantly upregulated in the HBR group compared with the HQR and BBR groups (P??0.05). Haem oxygenase (HMOX1) was significantly downregulated in BBR compared with HQR and HBR (P??0.05). Biliverdin reductase A (BLVRA), GUSB glucuronidase beta, cytochrome c-type haem lyase, protohaem IX farnesyltransferase and UGT2A2 were significantly upregulated in HBR and BBR compared with HQR (P??0.05). We conducted a comparative transcriptome analysis of the shell glands of Putian white ducks and Putian black ducks. None of the differentially regulated pathways were directly involved in the eggshell pigmentation process in the HQR-vs-HBR comparison, while 2 DEGs related to biliverdin breakdown were found in HQR-vs-BBR. Based on the RT-qPCR results, we can speculate that both HQR and HBR can produce biliverdin, but HBR cannot accumulate it. Compared with HQR, BBR produced less biliverdin and did not accumulate it.
机译：禽蛋壳颜色的多样性起着重要的生物作用，确保成功繁殖。蛋壳颜色也是家禽的重要特征，但在鸭子中潜在的机制是不知情的。本研究旨在为蓝绿蛋壳颜色发电机的机制提供见解。在这里，选用白壳鸭（HBr）和蓝绿壳鸭（HQR）选自莆田黑鸭，并选自莆田白鸭（BBR）。使用Illumina Hiseq 2500上的RNA测序分析壳体腺体中的转录变化。每图书馆测序二十七种单独的cDNA文库，平均每图书馆读取7.35亿读; 70.6％被映射到鸭参考基因组，平均检测到13,794个基因，其中占所有15,967个引燃鸭基因的约86.39％。在HQR和BBR基团之间检测总共899个差异表达基因（DEG），在HQR和HBR基团之间检测373℃。我们分析了HQR-VS-BBR和HQR-VS-HBR比较中的参数。这些DEG中的含量均未涉及HQR-VS-HBR中的蛋壳色素沉着方法，而UDP-葡糖醛糖基糖基转移酶2A2（UGT2A2）和UDP-葡糖醛糖基三烷基转移酶1-1-类似（UGT1-1类似），其参与Biliverdin分解，是HQR-VS-BBR中的两项参数。在RT-QPCR结果中，与HBR组与HBR和BBR基团相比，在HBR组中显着上调了δ-氨基乙酰丙酸合酶1（AlAs1）和EPRS谷氨酸 - 脯氨酰-TRNA合成酶（P？<β05）。与HQR和HBr相比，BBR中的碘氧酶（HMox1）显着下调（P？<β05）。 Biliverdin还原酶A（BLVRA），GUSB葡糖醛酸酶β，细胞色素C型血液裂解酶，PROHAEM IX法呢基转移酶和UGT2A2在HBr和BBR中显着上调，与HQR相比（P？<？0.05）。我们对莆田白鸭和莆田黑鸭的壳体进行了比较转录组分析。在HQR-VS-HBR比较中，没有差异调节的途径直接参与蛋壳色素沉着过程，而在HQR-VS-BBR中发现了与Biliverdin崩溃有关的2次。基于RT-QPCR结果，我们可以推测HQR和HBR均可产生Biliverdin，但HBR无法积累它。与HQR相比，BBR产生少于胆汁丁，并没有积累它。

著录项

相似文献

  • 外文文献
  • 中文文献
  • 专利
获取原文

客服邮箱：kefu@zhangqiaokeyan.com

京公网安备：11010802029741号 ICP备案号：京ICP备15016152号-6 六维联合信息科技 (北京) 有限公司©版权所有

  • 客服微信

  • 服务号