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首页> 外文期刊>International Journal of Molecular Sciences >Sweet Cherry ( Prunus avium L.) PaPIP1;4 Is a Functional Aquaporin Upregulated by Pre-Harvest Calcium Treatments that Prevent Cracking
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Sweet Cherry ( Prunus avium L.) PaPIP1;4 Is a Functional Aquaporin Upregulated by Pre-Harvest Calcium Treatments that Prevent Cracking

机译：甜樱桃（Prunus Avium L.）Papip1; 4是通过预收获钙治疗来上调的功能性水上蛋白，防止破裂

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The involvement of aquaporins in rain-induced sweet cherry ( Prunus avium L.) fruit cracking is an important research topic with potential agricultural applications. In the present study, we performed the functional characterization of PaPIP1;4, the most expressed aquaporin in sweet cherry fruit. Field experiments focused on the pre-harvest exogenous application to sweet cherry trees, cultivar Skeena, with a solution of 0.5% CaCl 2 , which is the most common treatment to prevent cracking. Results show that PaPIP1;4 was mostly expressed in the fruit peduncle, but its steady-state transcript levels were higher in fruits from CaCl 2 -treated plants than in controls. The transient expression of PaPIP1;4-GFP in tobacco epidermal cells and the overexpression of PaPIP1;4 in YSH1172 yeast mutation showed that PaPIP1;4 is a plasma membrane protein able to transport water and hydrogen peroxide. In this study, we characterized for the first time a plasma membrane sweet cherry aquaporin able to transport water and H 2 O 2 that is upregulated by the pre-harvest exogenous application of CaCl 2 supplements.
机译：Aquaporins在雨流诱导的甜樱桃（Prunus Avium L.）水果裂缝中的参与是具有潜在农业应用的重要研究课题。在本研究中，我们进行了Papip1的功能表征; 4，是甜樱桃果实中最表达的水素。田间实验主要集中在甜樱桃树，品种Skeena的预先收获外源性应用，0.5％CaCl 2的溶液，是最常见的治疗方法，以防止开裂。结果表明，Pap1; 4大部分在果梗中大多表达，但其稳态转录水平来自CaCl 2--治疗植物的水果比对照组。 Papip1; 4-GFP在烟草表皮细胞中的瞬时表达和PapiP1; 4在YSH1172酵母突变中显示出pap1; 4是能够运输水和过氧化氢的血浆膜蛋白。在这项研究中，我们首次表征了一种能够通过预先收获的CaCl 2补充剂进行水和H 2 O 2来运输水和H 2 O 2的素膜甜樱桃樱桃蛋白。

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