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A colorimetric nanoprobe based on enzyme-immobilized silver nanoparticles for the efficient detection of cholesterol

机译：基于酶固定化银纳米粒子的比色纳米孔用于有效检测胆固醇

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摘要

A large number of cardiovascular diseases have recently become of serious concern throughout the world. Herein, we developed a colorimetric probe based on functionalized silver nanoparticles (AgNPs) for the efficient sensing of cholesterol, an important cardiovascular risk marker. A simple sodium borohydride reduction method was employed to synthesize the AgNPs. The cholesterol oxidase (ChOx)-immobilized AgNPs interact with free cholesterol to produce H _(2) O _(2) in proportion to the concentration of cholesterol, resulting in decreased AgNP absorbance (turn-off) at 400 nm due to electron transfer between the AgNPs and H _(2) O _(2) . The response of the sensor can also be observed visually. The absorption intensity of the AgNPs is recovered (turn-on) upon the addition of sodium dodecyl sulfate due to the inhibition of ChOx. This on–off mechanism was effectively applied to detect cholesterol within the concentration range 10–250 nM with a low detection limit of approximately 0.014 nM. Moreover, the selectivity of the sensor toward cholesterol was analyzed in the presence of a range of interfering organic substances such as glucose, urea, and sucrose. Finally, the potential of the proposed sensor was evaluated using real samples.
机译：大量心血管疾病最近成为全世界严重关注。在此，我们开发了基于官能化银纳米颗粒（AgNP）的比色探针，以有效地感测胆固醇，这是一种重要的心血管风险标志物。使用简单的硼氢化钠还原方法合成AgNP。胆固醇氧化酶（CHOx） - 咪丙烯化酰胺与游离胆固醇相互作用，以与胆固醇的浓度成比例地产生H _（2）O _（2），导致由于电子转移导致400nm处的AgNP吸光度降低（关断） AgNP和H _（2）O _（2）之间。也可以在视觉上观察传感器的响应。由于抑制ChOx，在加入十二烷基硫酸钠时，回收AgNP的吸收强度（开启）。该开关机制有效地应用于检测10-250nm浓度范围内的胆固醇，低检测限为约0.014nm。此外，在一系列干涉有机物质如葡萄糖，尿素和蔗糖的存在下分析了传感器朝向胆固醇的选择性。最后，使用真实样品评估所提出的传感器的潜力。

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