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首页> 外文期刊>RSC Advances >Development and characterization of bladder acellular matrix cross-linked by dialdehyde carboxymethyl cellulose for bladder tissue engineering
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Development and characterization of bladder acellular matrix cross-linked by dialdehyde carboxymethyl cellulose for bladder tissue engineering

机译：膀胱羧甲基纤维素对膀胱组织工程的膀胱甲基纤维素交联的发展与鉴定

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In order to address the disadvantage of rapid degradation and serious immune response of bladder acellular matrix (BAM) tissues in clinical application, in this study, oxidized carboxymethyl cellulose (DCMC) was developed to replace glutaraldehyde (GA), a most common synthetic crosslinking reagent in clinical practice, to fix BAM tissues for lower cytotoxicity. The aim of this work was to evaluate feasibility of DCMC as a crosslinking reagent for BAM fixation and developing DCMC fixed-BAM (D-BAM) tissues for tissue engineering. For the preparation of DCMC, the results showed that when DCMC was prepared using a specific concentration of sodium periodate solution (the mass ratio of NaIO _(4) /CMC is 1?:?1) and a specific reaction time (4 hours), its cytotoxicity was the lowest and its fixation effect was better. The critical crosslinking characteristics and cytocompatibility of optimum D-BAM tissues were also investigated. The results demonstrated that DCMC-fixation (especially 30 mg ml ~(?1) DCMC-fixation) not only formed stable cross-linking bonds but also preserved well the original ultrastructure of the BAM tissues, which simultaneously increased the mechanical strength and capacity of the enzymatic hydrolytic resistance. The DCMC-fixation could also reduce the expression of α-Gal in BAM tissues and preserve the useful growth factors such as GAGs, KGF and TGF-β in bladder tissues. In addition, 30 mg ml ~(?1) D-BAM tissues had excellent cytocompatibility. Moreover, it could stimulate the secretion of PDGF and EGF from seeded bladder transitional epithelial cells (BTECs), which is a critical feature for further re-epithelialization. Its anti-calcification ability was also prominent, which is necessary in bladder repair. The present studies demonstrated that DCMC could be a potential biological crosslinking agent for BAM fixation due to its excellent crosslinking effects, and the D-BAM tissues were suitable to be used as a substitute for the bladder due to their resistance to enzymatic degradation, anticalcification and cytocompatibility.
机译：为了解决临床应用中膀胱细胞胚胎基质（BAM）组织快速降解和严重免疫应答的缺点，在本研究中，开发了氧化羧甲基纤维素（DCMC）以取代戊二醛（GA），是最常见的合成交联试剂在临床实践中，固定较低细胞毒性的BAM组织。这项工作的目的是评估DCMC作为用于BAM固定的交联试剂和显影组织工程的DCMC固定BAM（D-BAM）组织的可行性。为了制备DCMC，结果表明，当使用特定浓度的钠碘酸钠溶液制备DCMC时（NaiO _（4）/ cmc的质量比为1？：1）和特定的反应时间（4小时） ，其细胞毒性是最低的，其固定效果更好。还研究了最佳的D-BAM组织的临界交联特性和细胞锁定性。结果表明，DCMC固定（特别是30mg mL〜（α1）DCMC固定）不仅形成了稳定的交联键，而且还保留了BAM组织的原始超微结构，同时增加了机械强度和容量酶水解抗性。 DCMC固定还可以降低BAM组织中α-GAL的表达，并保留膀胱组织中的有用生长因子，如GAG，KGF和TGF-β。另外，30mg mL〜（α1）D-BAM组织具有优异的细胞织组种。此外，它可以刺激来自种子膀胱移行上皮细胞（BTEC）的PDGF和EGF的分泌，这是进一步重新上皮化的关键特征。它的抗钙化能力也突出，这在膀胱修复中是必要的。本研究证明，由于其优异的交联效应，DCMC可以是用于BAM固定的潜在的生物交联剂，并且由于它们对酶促降解，抗核化和抗性和抗粘液的抵抗力，D-BAM组织适合用作膀胱的替代物细胞势杂性。

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