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首页> 外文期刊>RSC Advances >Fused pyrazole-phenanthridine based dyads: synthesis, photo-physical and theoretical studies, and live cell pH imaging
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Fused pyrazole-phenanthridine based dyads: synthesis, photo-physical and theoretical studies, and live cell pH imaging

机译：融合吡唑-菲利啶的二元：合成，照片物理和理论研究，和活细胞pH成像

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摘要

The arrangement of small sized molecules with a scaffold structure plays an active role in the fields of sensors and health care. An efficient molecular design strategy for four pyrazole-phenanthridine based D–π–A luminophores, denoted as 2a , 2b , 2c and 2d was developed to investigate the effect of acid on the photo-physical properties of these dyes. Photo-physical studies of the synthesized probes showed distinct absorption and emission under various pH conditions. Theoretical calculations using density functional methods were carried out for understanding the mechanistic aspects of the proton induced fluorescence. The experimentally observed photo-physical properties correlated well with theoretical results. Moreover, probes 2 and 2a can be used to monitor the fluorescence changes in E. coli cells under different pH conditions.
机译：具有脚手架结构的小型分子的布置在传感器和医疗保健领域中起着积极作用。开发了一种基于四个吡唑 - 菲利啶的D-π-A发光体的有效分子设计策略，表示为2A，2B，2C和2D，以研究酸对这些染料的光学性质的作用。合成探针的照片物理研究表明在各种pH条件下的显着吸收和发射。进行了使用密度官能化方法的理论计算，以了解质子诱导荧光的机械方面。实验观察到的光学物理性质与理论结果相关。此外，探针2和2a可用于在不同pH条件下监测大肠杆菌细胞中的荧光变化。

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